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- W3147013755 abstract "Objective To explore whether recombinant adeno-associated virus mediated antisense vascular endothelial growth factor (rAAV-aVEGF) gene transfer inhibits the development of corneal neovascularization (CNV) in a rat model. Methods rAAV-aVEGF165 and recombinant adeno-associated virus mediated Lacz (rAAV-Lacz) were constructed by three-plasmid contransfection methods. Forty-eight Sprague-Dawley (SD) rats were divided into two groups randomly. CNV were induced in vivo by alkaline cauterization of the central cornea. Twenty-four rats were injected with rAAV-aVEGF 165 (10 10 pfu/ml) into conjunctiva; another rats were injected with rAAV-Lacz (10 10 pfu/ml) into conjunctiva as controls. The Lacz gene expression was evaluated by 5-bromo-4-chloro-3-indolyl-β-D-galactoside (X-gal) immunohistochemical staining. 1 to 30 days post-cautery, CNV was evaluated by morphometric analysis, and expression of VEGF was evaluated by immunhistochemistry. Results Two days later, 21.36%±1.07% Lacz gene expression was detected in conjunctival sac in control group. 30 d later, 28.02%±1.16% Lacz gene remained expression. Morphometric analysis and immunhistochemistry demonstrated rAAV-mediated antisense VEGF 165 significantly inhibited CNV. The VEGF 165 protein was decreased post-cautery compared to control group injected with. rAAV-Lacz(F=1639.22,F=2187.16,F=719.17, P0.01). Conclusions This study suggests that rAAV-aVEGF 165 can sufficiently inhibit the cautery-induced CNV, and the effect is associated with the inhibition of VEGF production." @default.
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- W3147013755 date "2006-01-01" @default.
- W3147013755 modified "2023-09-23" @default.
- W3147013755 title "Antisense vascular endothelial growth factor suppressed corneal neovascularization in rats" @default.
- W3147013755 hasPublicationYear "2006" @default.
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