FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W3147025181> ?p ?o ?g. }

• W3147025181 abstract "Background: Gene targeting in vivo provides a potentially powerful method for gene analysis and gene therapy. In order to sensitively detect and accurately measure designed sequence changes, we have used a transgenic mouse system, MutaMouse, which has been developed for detection of mutation in vivo. It carries bacteriophage lambda genome with lacZ+ gene, whose change to lacZnegative allele is detected after in vitro packaging into bacteriophage particles. We have also demonstrated that gene transfer with a replication-defective adenovirus vector can achieve efficient and accurate gene targeting in vitro. Methods: An 8 kb long DNA corresponding to the bacteriophage lambda transgene with one of two lacZ-negative single-base-pair-substitution mutant allele was inserted into a replicationdefective adenovirus vector. This recombinant adenovirus was injected to the transgenic mice via tail-vein. Twenty-four hours later, genomic DNA was extracted from the liver tissue and the lambda::lacZ were recovered by in vitro packaging. The lacZ-negative phage was detected as a plaque former on agar with phenyl-beta-D-galactoside. Results: The mutant frequency of the lacZ-negative recombinant adenovirus injected mice was at the same level with the control mouse (~1/10000). Our further restriction analysis did not detect any designed recombinant. Conclusion: The frequency of gene targeting in the mouse liver by these recombinant adenoviruses was shown to be less than 1/20000 in our assay. However, these results will aid the development of a sensitive, reliable and PCR-independent assay for gene targeting in vivo mediated by virus vectors and other means. Background Gene targeting, which is the precise alteration of genomic information by homologous recombination, has provided a powerful means of genetic analysis in Published: 12 October 2005 Genetic Vaccines and Therapy 2005, 3:8 doi:10.1186/1479-0556-3-8 Received: 01 July 2005 Accepted: 12 October 2005 This article is available from: http://www.gvt-journal.com/content/3/1/8 © 2005 Ino et al; licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited." @default.
• W3147025181 created "2021-04-13" @default.
• W3147025181 creator A5002392148 @default.
• W3147025181 creator A5022775273 @default.
• W3147025181 creator A5034262525 @default.
• W3147025181 creator A5072872846 @default.
• W3147025181 creator A5086287691 @default.
• W3147025181 creator A5087006540 @default.
• W3147025181 date "2015-01-01" @default.
• W3147025181 modified "2023-10-02" @default.
• W3147025181 title "Genetic Vaccines and Therapy" @default.
• W3147025181 cites W1519638472 @default.
• W3147025181 cites W1557986375 @default.
• W3147025181 cites W1590783856 @default.
• W3147025181 cites W1802529537 @default.
• W3147025181 cites W1820036906 @default.
• W3147025181 cites W1900784661 @default.
• W3147025181 cites W1913924834 @default.
• W3147025181 cites W1914977630 @default.
• W3147025181 cites W1969405745 @default.
• W3147025181 cites W1973919938 @default.
• W3147025181 cites W1976856900 @default.
• W3147025181 cites W1982057521 @default.
• W3147025181 cites W1982444756 @default.
• W3147025181 cites W1984640298 @default.
• W3147025181 cites W1994428481 @default.
• W3147025181 cites W1994639228 @default.
• W3147025181 cites W2004184676 @default.
• W3147025181 cites W2007973509 @default.
• W3147025181 cites W2016497543 @default.
• W3147025181 cites W2017271426 @default.
• W3147025181 cites W2017310256 @default.
• W3147025181 cites W2020101522 @default.
• W3147025181 cites W2020389017 @default.
• W3147025181 cites W2024204233 @default.
• W3147025181 cites W2026109158 @default.
• W3147025181 cites W2026544727 @default.
• W3147025181 cites W2028622989 @default.
• W3147025181 cites W2028930741 @default.
• W3147025181 cites W2038277900 @default.
• W3147025181 cites W2040149948 @default.
• W3147025181 cites W2045973564 @default.
• W3147025181 cites W2048585851 @default.
• W3147025181 cites W2063042784 @default.
• W3147025181 cites W2073851975 @default.
• W3147025181 cites W2086270776 @default.
• W3147025181 cites W2092806325 @default.
• W3147025181 cites W2093465243 @default.
• W3147025181 cites W2095418304 @default.
• W3147025181 cites W2106357210 @default.
• W3147025181 cites W2113074370 @default.
• W3147025181 cites W2133775313 @default.
• W3147025181 cites W2134441159 @default.
• W3147025181 cites W2136104310 @default.
• W3147025181 cites W2141818408 @default.
• W3147025181 cites W2151988588 @default.
• W3147025181 cites W2156077817 @default.
• W3147025181 cites W2160037336 @default.
• W3147025181 cites W2167241103 @default.
• W3147025181 cites W2170184906 @default.
• W3147025181 cites W2337234078 @default.
• W3147025181 cites W2464357126 @default.
• W3147025181 hasPublicationYear "2015" @default.
• W3147025181 type Work @default.
• W3147025181 sameAs 3147025181 @default.
• W3147025181 citedByCount "0" @default.
• W3147025181 crossrefType "journal-article" @default.
• W3147025181 hasAuthorship W3147025181A5002392148 @default.
• W3147025181 hasAuthorship W3147025181A5022775273 @default.
• W3147025181 hasAuthorship W3147025181A5034262525 @default.
• W3147025181 hasAuthorship W3147025181A5072872846 @default.
• W3147025181 hasAuthorship W3147025181A5086287691 @default.
• W3147025181 hasAuthorship W3147025181A5087006540 @default.
• W3147025181 hasConcept C102230213 @default.
• W3147025181 hasConcept C104317684 @default.
• W3147025181 hasConcept C111599444 @default.
• W3147025181 hasConcept C143065580 @default.
• W3147025181 hasConcept C153911025 @default.
• W3147025181 hasConcept C159047783 @default.
• W3147025181 hasConcept C170344550 @default.
• W3147025181 hasConcept C207001950 @default.
• W3147025181 hasConcept C2776441376 @default.
• W3147025181 hasConcept C32470452 @default.
• W3147025181 hasConcept C40767141 @default.
• W3147025181 hasConcept C43953973 @default.
• W3147025181 hasConcept C501734568 @default.
• W3147025181 hasConcept C54355233 @default.
• W3147025181 hasConcept C547475151 @default.
• W3147025181 hasConcept C86803240 @default.
• W3147025181 hasConceptScore W3147025181C102230213 @default.
• W3147025181 hasConceptScore W3147025181C104317684 @default.
• W3147025181 hasConceptScore W3147025181C111599444 @default.
• W3147025181 hasConceptScore W3147025181C143065580 @default.
• W3147025181 hasConceptScore W3147025181C153911025 @default.
• W3147025181 hasConceptScore W3147025181C159047783 @default.
• W3147025181 hasConceptScore W3147025181C170344550 @default.
• W3147025181 hasConceptScore W3147025181C207001950 @default.
• W3147025181 hasConceptScore W3147025181C2776441376 @default.
• W3147025181 hasConceptScore W3147025181C32470452 @default.
• W3147025181 hasConceptScore W3147025181C40767141 @default.

• next