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- W3147134243 abstract "Temperature is one of the key environmental factors influencing the viability of C.parvum.Assessment of risks of outbreaks requires valid methods for accurate evaluation of oocyst viability.Using nucleic acid stain and mouse infectivity methods the present work investigated viability of intact and partial oocysts post excystation and heat treatment and compared to that of fresh oocysts.Intact oocyst post excystation or treatment at moderate temperatures remained viability.However,intact oocysts post heat treatment at high temperatures and partial oocysts post excystation or heat-treatments lost viability completely.The SYTO-59 stain parallels with mouse infectivity on fresh oocysts,inactivated oocysts,partial oocysts,and intact oocysts post excystation and treatment at 40℃ and 70℃ but not on intact oocysts post treatment at 50℃ and 60℃.Findings from the present study provide a knowledge and technical information for properly and precisely risk assessment of waterborne C.parvum." @default.
- W3147134243 created "2021-04-13" @default.
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- W3147134243 date "2009-01-01" @default.
- W3147134243 modified "2023-09-25" @default.
- W3147134243 title "Viability of Cryptosporidium parvum oocysts post excystation and heat treatment assessed by nucleic acid stain and mouse infectivity" @default.
- W3147134243 hasPublicationYear "2009" @default.
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