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- W3147258313 abstract "【Objective】The effects of insulin-like growth factor-I(IGF-I) on the quality and fertility of cryopreservation ovine semen were evaluated to elucidate the optimal IGF-I concentration and increase ovine fertility rate and accelerate breed improvement. 【Method】Sperm ejaculates from Texel rams were diluted in a glucose-sodium citrate extender of IGF-I doses tested(0,50,100,150 and 250 ng/m L). Sperm viability and acrosome integrity, plasma membrane integrity and DNA integrity were assessed after 0,1 and 2 h thawing as well as in vitro fertility. 【Result】The results showed that after 0,1 and 2 h thawing, sperm viability, acrosome integrity and DNA integrity were higher in groupⅡ(experimental extenders containing IGF-Ι 100 ng/m L) compared with other groups. With the time prolonged, the sperm acrosome integrity gradually decreased. Regarding the sperm plasma membrane and DNA integrity, group Ⅱ was higher than other groups. Shorter incubation time(1 h) meant more obvious effect, which was significantly higher than the control group(P0.05). In vitro fertility rate and blastocyst rate was no difference(P0.05) among the five experimental groups, and the effect of in vitro fertility of group Ⅱoutdid other groups. 【Conclusion 】IGF-I helps to improve subjective sperm viability, survival rate of cryopreserved semen and maintain structural integrity of plasma membrane,acrosome and DNA if relevant procedures of frozen-thawed ovine semen can be completed within an hour." @default.
- W3147258313 created "2021-04-13" @default.
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- W3147258313 date "2015-01-01" @default.
- W3147258313 modified "2023-09-23" @default.
- W3147258313 title "Effects of IGF-Ι on quality traits of sheep cryopreserved ovine semen" @default.
- W3147258313 hasPublicationYear "2015" @default.
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