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- W3147260685 abstract "Objectives To isolate and identify novel genes of Schistosoma japonicum(S.j) from cDNA library. Methods cDNA clones were screened from S.j cDNA library by immunological method, the inserts of positive cDNA clones were identified by PCR directed sequencing and homologous searching of EST, the cDNA sequence encoding S.j arginase was analyzed by the subcloning and bioinformatics. Results The positive cDNA clone with 1 061 bp insert was isolated from S.j cDNA library, which sequence of the insert included a ORF with 840 bp, and translated into 279 amino acid residues. The deduced protein was similar to the arginase family, the homology at amino acid sequence level was 44%,50%,51%,53% and 54% with schizosacch aromyces pombe (fission yeast) arginase, xenopus laevis (african clawed frog) arginase, homo sapiens (human) arginase ii precursor and rattus norvegicus (rat) arginase ii precursor respectively. Conclusion The full length cDNA sequence encoding Schistosoma japonicum arginase was cloned, which gave the basis for further studying of Schistosoma japonicum arginase." @default.
- W3147260685 created "2021-04-13" @default.
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- W3147260685 date "2001-01-01" @default.
- W3147260685 modified "2023-09-25" @default.
- W3147260685 title "Isolation and Sequence Analysis of the cDNA Encoding Schistosoma japonicum Arginase" @default.
- W3147260685 hasPublicationYear "2001" @default.
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