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- W3147373695 abstract "Publisher Summary This chapter discusses the methods for standard genetic analyses in Tetrahymena thermophila. A method for complementation testing is presented by which any two alleles—whether dominant or recessive—can be analyzed. Genetic analyses in Tetrahymena can be accomplished by a number of diverse strategies. Using separate approaches, it is possible to interrogate either the micronuclear or the macronuclear genome. For micronuclear analysis, cells can be mated to diploid marker strains for conventional genetic linkage, segregation, and complementation tests. In addition, new mutant lines can be mated to cells that carry micro- and macronuclei with different genotypes (genetic heterokaryons) for mapping. Crosses can also be performed to a unique set of strains (the star strains) which have in common the inability to transfer pronuclei to partners in crosses; matings to these strains induce a unique form of self-mating, termed genomic exclusion. These matings yield whole genome homozygous micronuclei and either parental or identical homozygous macronuclei." @default.
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- W3147373695 date "1999-01-01" @default.
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- W3147373695 title "Chapter 7 Methods for Genetic Analysis" @default.
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- W3147373695 doi "https://doi.org/10.1016/s0091-679x(08)61533-2" @default.
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