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- W3147383269 abstract "到分子的诊断的技术保持大的聚合酶链反应(PCR ) 的申请为农业上的早鉴定的诺言重要植物病原体。Ralstonia solanacearum， Xanthomoans axonopodis pv。vesicatoria，和 Xanthomonas oryzae pv。oryzae 是细菌，能感染蔬菜，引起严重收益损失的 phytopathogenic。PCR-single-strand 符合构造多型性(PCR-SSCP ) 是为在放大 DNA 识别顺序变化的一种简单、强大的技术。PCR-SSCP 的技术到目前为止正在被利用，到仅仅除了植物检测并且诊断人的细菌的病原体病原的真菌。选择媒介和血清学是为在感染的植物材料的植物病原体的察觉的通常使用的方法。在这研究，我们开发了 PCR-SSCP 技术识别 phytopathogenic 细菌。PCR 产品在非使中毒的 polyacrylamide 胶化上被使中毒并且分开。SSCP banding 模式被银染色 nucleic 酸检测。我们测试了超过 56 R 孤立。solanacearum， 44 X 孤立。axonopodis pv。vesicatoria，并且 20 X 孤立。oryzae pv。oryzae。与通用教材 16S rRNA 的使用，我们能在类和种类层次区别如此的种类。种类特定的模式为细菌 R 被获得。solanacearum， X。axonopodis pv。vesicatoria，和 X。oryzae pv。oryzae。为 phytobacterial 病原体的察觉和诊断的 PCR-SSCP 技术的潜在的使用在现在的纸被讨论。" @default.
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- W3147383269 date "2012-01-01" @default.
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- W3147383269 title "Molecular detection of plant pathogenic bacteria using polymerase chain reaction single-strand conformation polymorphism" @default.
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