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- W3147394911 abstract "我们构造了并且描绘，并且获得了从 431 鈥的 905 个 nonredundant 序列(NRS ) Nannochloropsis oculata CS-179 的一个规范的 cDNA 图书馆吗？在长度的 756 bp。在他们之中， 496 很类似于在 GenBank (E 鈮 ?.0e-05 ) 的 nonredundant，并且 349 EST 与真核细胞的 orthologous 组(KOG ) 的簇有重要点击。在 14 氨基酸残余的 codons 的第三个位置的底 G 或 C 在 362 NRS 的保存领域建议了强壮的偏爱(>60%) 。我们也识别了编码磷和氮 transporters 的 unigenes，建议那 N。oculata 能高效地搬运并且使产生代射变化磷和氮，并且认出了在丰满的酸和多元不堡和的生合成和存储包含了丰满的酸(PUFA ) 的 unigenes，它将便于 eicosapentaenoic 酸(EPA ) 的示范 N 的生合成小径。oculata。与原来的 cDNA 图书馆比较，规范的图书馆显著地增加了随机的定序的效率并且很少表示了发现的基因，并且减少丰富的基因序列的频率。关键词 Eustigmatophyceae - Nannochloropsis oculata - 规范的 cDNA 图书馆 - 国家关键技术 R&D 节目在中国(号码 2006BAD09A03-2 ) 和国家高技术研究和中国(863 程序)( 号码 2007AA09Z427 ) 的开发的 11th-Five-Year 计划支持的表示顺序标签" @default.
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- W3147394911 date "2010-01-01" @default.
- W3147394911 modified "2023-09-24" @default.
- W3147394911 title "Construction and characterization of a normalized cDNA library of Nannochloropsis oculata（Eustigmatophyceae）" @default.
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