Matches in SemOpenAlex for { <https://semopenalex.org/work/W3147395410> ?p ?o ?g. }
Showing items 1 to 78 of
78
with 100 items per page.
- W3147395410 abstract "In this study, we characterized the bioenergetic response of the Lund human mesencephalic (LUHMES) cell line and a mouse astrocyte cell line to oxidative stress. Extracellular hydrogen peroxide (H2O2) levels and bioenergetic response were investigated in these cell lines after exposure to paraquat (PQ), a redox cycling compound that causes oxidative stress in cells. We used extracellular flux analysis to measure mitochondrial function in adherent astrocytes and LUHMES cells. Extracellular H2O2 was measured fluorometrically. H2O2 levels increased in both cell lines after exposure to 5 µM PQ for 18 h; however, the extent of H2O2 increase with astrocytes was significantly lower than that with LUHMES cells (33% vs. 67%). Measurements of basal mitochondrial respiration showed that PQ almost completely eliminated oxygen consumption rate (OCR) in astrocytes and significantly reduced it in LUHMES cells. Notably, OCR in LUHMES cells was higher than that in astrocytes, indicating that neuronal cells maintain higher oxidative metabolism than glial cells, which is also consistent with higher energy demands of the neuronal cells. Moreover, LUHMES cells exhibited a higher amount of adenosine triphosphate (ATP) being produced by oxidative phosphorylation than by glycolysis. In contrast, astrocytes demonstrated a higher glycolytic capacity and glycolytic reserve than LUHMES cells and higher ATP production rate by glycolysis than its production by mitochondrial oxidative metabolism. Collectively, this study showed the differential bioenergetic responses between astrocytes and LUHMES cells in responding to oxidative stress and the findings may provide insights into the mitochondrial reserve capacity in neurons and astrocytes in responding to oxidative stress. (First online: Mar 30, 2021)" @default.
- W3147395410 created "2021-04-13" @default.
- W3147395410 creator A5021114754 @default.
- W3147395410 creator A5025884894 @default.
- W3147395410 creator A5044609569 @default.
- W3147395410 creator A5064440739 @default.
- W3147395410 creator A5078715438 @default.
- W3147395410 date "2021-03-30" @default.
- W3147395410 modified "2023-10-07" @default.
- W3147395410 title "Assessing Bioenergetic Function in Response to Reactive Oxygen Species in Neural Cells" @default.
- W3147395410 doi "https://doi.org/10.20455/ros.2021.r.803" @default.
- W3147395410 hasPublicationYear "2021" @default.
- W3147395410 type Work @default.
- W3147395410 sameAs 3147395410 @default.
- W3147395410 citedByCount "1" @default.
- W3147395410 countsByYear W31473954102022 @default.
- W3147395410 crossrefType "journal-article" @default.
- W3147395410 hasAuthorship W3147395410A5021114754 @default.
- W3147395410 hasAuthorship W3147395410A5025884894 @default.
- W3147395410 hasAuthorship W3147395410A5044609569 @default.
- W3147395410 hasAuthorship W3147395410A5064440739 @default.
- W3147395410 hasAuthorship W3147395410A5078715438 @default.
- W3147395410 hasConcept C100206155 @default.
- W3147395410 hasConcept C134018914 @default.
- W3147395410 hasConcept C185592680 @default.
- W3147395410 hasConcept C20251656 @default.
- W3147395410 hasConcept C2776151105 @default.
- W3147395410 hasConcept C2777542381 @default.
- W3147395410 hasConcept C2779564974 @default.
- W3147395410 hasConcept C28406088 @default.
- W3147395410 hasConcept C28859421 @default.
- W3147395410 hasConcept C48349386 @default.
- W3147395410 hasConcept C529278444 @default.
- W3147395410 hasConcept C54355233 @default.
- W3147395410 hasConcept C55493867 @default.
- W3147395410 hasConcept C57600042 @default.
- W3147395410 hasConcept C62231903 @default.
- W3147395410 hasConcept C66517615 @default.
- W3147395410 hasConcept C81885089 @default.
- W3147395410 hasConcept C86803240 @default.
- W3147395410 hasConcept C95444343 @default.
- W3147395410 hasConceptScore W3147395410C100206155 @default.
- W3147395410 hasConceptScore W3147395410C134018914 @default.
- W3147395410 hasConceptScore W3147395410C185592680 @default.
- W3147395410 hasConceptScore W3147395410C20251656 @default.
- W3147395410 hasConceptScore W3147395410C2776151105 @default.
- W3147395410 hasConceptScore W3147395410C2777542381 @default.
- W3147395410 hasConceptScore W3147395410C2779564974 @default.
- W3147395410 hasConceptScore W3147395410C28406088 @default.
- W3147395410 hasConceptScore W3147395410C28859421 @default.
- W3147395410 hasConceptScore W3147395410C48349386 @default.
- W3147395410 hasConceptScore W3147395410C529278444 @default.
- W3147395410 hasConceptScore W3147395410C54355233 @default.
- W3147395410 hasConceptScore W3147395410C55493867 @default.
- W3147395410 hasConceptScore W3147395410C57600042 @default.
- W3147395410 hasConceptScore W3147395410C62231903 @default.
- W3147395410 hasConceptScore W3147395410C66517615 @default.
- W3147395410 hasConceptScore W3147395410C81885089 @default.
- W3147395410 hasConceptScore W3147395410C86803240 @default.
- W3147395410 hasConceptScore W3147395410C95444343 @default.
- W3147395410 hasLocation W31473954101 @default.
- W3147395410 hasOpenAccess W3147395410 @default.
- W3147395410 hasPrimaryLocation W31473954101 @default.
- W3147395410 hasRelatedWork W1979451357 @default.
- W3147395410 hasRelatedWork W2012533194 @default.
- W3147395410 hasRelatedWork W2027088686 @default.
- W3147395410 hasRelatedWork W2038365047 @default.
- W3147395410 hasRelatedWork W2097466887 @default.
- W3147395410 hasRelatedWork W2115021528 @default.
- W3147395410 hasRelatedWork W2133486230 @default.
- W3147395410 hasRelatedWork W2525625242 @default.
- W3147395410 hasRelatedWork W2903829469 @default.
- W3147395410 hasRelatedWork W3147395410 @default.
- W3147395410 hasVolume "11" @default.
- W3147395410 isParatext "false" @default.
- W3147395410 isRetracted "false" @default.
- W3147395410 magId "3147395410" @default.
- W3147395410 workType "article" @default.