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- W3147444838 abstract "Using cDNA representational difference analysis (cDNA RDA) method, we have successfully isolated a gene fragment whose expression was specifically induced by external GAa application. Screening a G2 pea cDNA library using this fragment as a probe, we obtained a 2036 bp full-length cDNA. It contains a 1746 bp open reading frame and encodes a protein of 581 amino acids with a theoretical molecular weight of 64 ku. It shares high-level sequenceidentity with AAIR genes from other plant species. This cDNA was cloned into expression vector and recombinant E. coli DH5α cells with remarkable AAIR enzyme activity were obtained." @default.
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- W3147444838 date "2001-01-01" @default.
- W3147444838 modified "2023-09-27" @default.
- W3147444838 title "Cloning and analysis of a cDNA encoding acetohydroxy acid isomeroreductase from G2 pea" @default.
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