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- W3147461144 abstract "RT–PCR were used to investigate the expression of Atsb3 in Arabidopsis thaliana, and the vector with AtSb3gene promoter and GUS fusion expression cassette was constructed to obtain the transgenic lines of Arabidopsis thaliana,based on which the tissue localization of AtSb3 gene expression was investigated by histochemical staining. The results of RT–PCR showed AtSb3 gene was expressed only in root, not in the stem, leaf, flower and silique. The results of GUS staining showed that in seeds at early germinating stage,AtSb3 gene was only expressed in radicle. In cotyledon expansion period after seed germination, AtSb3 gene expression was only detected in root tip, not in other parts of the plant. In 4 to 5 leaf stage seedling,AtSb3 was not expressed in aboveground parts but in taproots and lateral roots. In the root tip, GUS staining was heavier in conjunction region of the root cap and meristematic zone, as well as in elongation zone and root hair, which indicated expression level of AtSb3 gene was higher in these parts; GUS staining in the region between meristematic zone and elongation zone was not observed." @default.
- W3147461144 created "2021-04-13" @default.
- W3147461144 creator A5012390171 @default.
- W3147461144 date "2013-01-01" @default.
- W3147461144 modified "2023-09-23" @default.
- W3147461144 title "Analysis of the expression of AtSb3 gene containing MATH domains in Arabidopsis thaliana" @default.
- W3147461144 hasPublicationYear "2013" @default.
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