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- W3147584482 abstract "In order to explore PVY resistance-related gene and further reveal its resistance mechanism, the tobacco mutants SN01 and SN02 highly resistant to potato virus Y vein necrosis strain(PVYN) were experimented to study the role of translation initiation factor e IF4 E of PVY resistance-related gene in the mutants. The results showed that full length target genes were successfully cloned from the mutants SN01 and SN02. The genes were referred to as e IF4E01 and e IF4E02, respectively; their open reading frame(ORF) was 669 bp in length and encoded a protein of 222 amino acids. The homology analysis by MEGA 4.1 and CLUSTAL showed that these genes possessed 99% sequence similarities to and exactly the same ORF sequence as reported e IF4 E in tobacco(Gen Bank: AY702653.1), they were highly homologous to the e IF4 E family genes in Capsicum annuum, Lycopersicon esculentum and Arabidopsis thaliana. Real-time PCR results indicated that the expression level of e IF4 E in the mutants was obviously lower thanthat in their susceptible parents NC89 and K326 at the early stage of PVYNinfection(0-9 days after inoculation), it indicated that the suppression of e IF4 E expression in the two mutants was closely related to the resistance of tobacco to PVYN." @default.
- W3147584482 created "2021-04-13" @default.
- W3147584482 creator A5019803256 @default.
- W3147584482 date "2015-01-01" @default.
- W3147584482 modified "2023-09-23" @default.
- W3147584482 title "Cloning and Expression Analysis of e IF4E Gene in Tobacco Mutants Resistant to PVY~N" @default.
- W3147584482 hasPublicationYear "2015" @default.
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