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- W3147601092 abstract "盐十字花科植物(Thellungiella halophila ) ，模型的近亲种 Arabidopsis thaliana L. ，是被使适应严厉盐的环境的 extremophile。到我的从这种的咸忍耐的基因，我们从对待与的盐十字花科植物工厂构造了一个入口 cDNA 图书馆由使用修改 cDNA 合成和完全没有操作正在包含限制酶和脱氧核糖核酸连接酶的一个改进帮助再结合的 cDNA 图书馆构造方法咸压力。这个 cDNA 图书馆构造过程显著地被简化， cDNA 图书馆的质量被改进。这个入口 cDNA 图书馆是随后，进目的地二进制代码向量 pCB406 的 shuttled 经由帮助再结合的克隆为工厂转变和表示设计了。图书馆是准备好了的工厂转变并且被用来在大规模上转变 Arabidopsis 以便为功能的基因采矿创造转基因的线的一个大集合。" @default.
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- W3147601092 date "2007-01-01" @default.
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- W3147601092 title "Construction of a Plant Transformation-ready Expression cDNA Library for Thellungiella halophila Using Recombination Cloning" @default.
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