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- W3147604436 abstract "Abstract. Abass A, Mosi L, Gbewonyo WSK. 2018. Evaluation of antimycobacterial and antimycolactone efficaciousness of kombucha. Bioteknologi 15: 80-91. This study was intended at distinguishing the microbial substance of kombucha tea, appraising kombucha antimicrobial, antimycobacterial and mycolactone deactivating potential. The antioxidants, as well as the phytochemical constituents of the tea, were also examined. Kombucha tea was cultured, and microbial isolates were Gram stained, DNA extracted, followed by PCR with 16S rRNA and fungal ITS primers for recognition of bacteria and yeasts, successively. Resulting amplicons were sequenced and blasted against the PUBMED database for recognition based on sequence alikeness and homology. Phytochemical analysis was carried out on the kombucha tea and the total phenolic substances were ascertained. DPPH assay was utilized for the ascertainment of the antioxidant characteristic of the tea. Dissimilar concentrations of the tea were co-brooded with mycolactone for numerous time points to examine for poison weakening. The existence of undamaged or deactivated mycolactone was discovered utilizing TLC and cytotoxicity assays on cultured human fibroblast cell lines. Antimicrobial efficacy of elevated concentrations of the tea was tried out against S. aureus and M. ulcerans by pre-hatching before microscopy and culture to examine morphological alterations and survival successively. Yeasts in the kombucha tea were recognized as Dekkera bruxellensis, Brettanomyces bruxellensis, Rhodotorula mucilaginosa and Lachancea fermentati and the bacteria as Paenibacillus lactis, Paenibacillus cineris Bacillus licheniformis, Lactobacillus amylolyticus and Corynebacterium glutamic. Phytochemicals discovered in the tea were saponins, flavonoids, alkaloids, phenols and terpenoids. The antioxidant characteristic of kombucha was higher contrasted with the fresh tea and a resemblant impact was perceived with the phenolic substance. Fresh was 2-fold less efficacious in total phenolic substance than kombucha tea. The tea had antimicrobial liveliness against S. aureus but not on M. ulcerans. Kombucha was not cytotoxic to the human skin fibroblast cells, however, mycolactone administered with kombucha tea preserved its efficacy against human fibroblast cell lines and sustainable bacilli of M. ulcerans were perceived after kombucha medication. Kombucha tea has antioxidant and antimicrobial livelinesses but lacks antimycobacterial and antimycolactone liveliness." @default.
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- W3147604436 date "2018-01-01" @default.
- W3147604436 modified "2023-09-23" @default.
- W3147604436 title "Evaluation of the antimycobacterial and antimycolactone efficaciousness of kombucha" @default.
- W3147604436 doi "https://doi.org/10.13057/biotek/c150204" @default.
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