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- W3147618879 abstract "The NADPH-dependent aldehyde reductase gene was cloned from the genome of Sporobolomyces salmonicolor ZJU0105,and the recombinant E.coli BL21(pET28-ALR0105) strain was constructed.The asymmetric reduction of ethyl 4-chloroacetoacetate(COBE) to ethyl(R)-(+)-4-chloro-3-hydroxybutyrate((R)-CHBE) by the recombinant cells in an aqueous phase was investigated.The product is optically pure(R)-CHBE,and both the(R)-CHBE yield and stereoselectivity are higher than those in the reaction catalyzed by Sporobolomyces salmonicolor.The glucose dehydrogenase,NADPH and glucose are required for the recombinant cells to regenerate NADPH.The reaction is also inhibited by high concentration of COBE and CHBE,but high density of recombinant cells can decrease this inhibitory effect.A yield of 96.5% and ee of 99% for(R)-CHBE are obtained under the appropriate reaction conditions." @default.
- W3147618879 created "2021-04-13" @default.
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- W3147618879 date "2005-01-01" @default.
- W3147618879 modified "2023-09-25" @default.
- W3147618879 title "Asymmetric Reduction of Ethyl 4-Chloroacetoacetate to Ethyl (R)-(+)-4-Chloro-3-Hydroxybutyrate by Recombinant Escherichia coli" @default.
- W3147618879 hasPublicationYear "2005" @default.
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