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• W3147639680 endingPage "103795" @default.
• W3147639680 startingPage "103795" @default.
• W3147639680 abstract "Broiler meat is considered as the most important source of the foodborne pathogen Campylobacter jejuni . Exposure to stress conditions encountered during the slaughtering process may induce bacterial adaptation mechanisms, and enhance or decrease pathogen resistance to subsequent stress. This adaptation may result from changes in bacterial gene expression. This study aims to accurately quantify the expression of selected C. jejuni genes after stresses inspired from the poultry slaughtering process. RT-qPCR was used to quantify gene expression of 44 genes in three strains after successive heat and cold stresses. Main results indicated that 26 genes out of 44 were differentially expressed following the successive thermal stresses. Three clusters of genes were differentially expressed according to the strain and the stress condition. Up-regulated genes mainly included genes involved in the heat shock response, whereas down-regulated genes belonged to metabolic pathways (such as lipid, amino-acid metabolisms). However, four genes were similarly overexpressed in the three strains; they might represent indicators of the thermal stress response at the species scale. Advances in the molecular understanding of the stress response of pathogenic bacteria, such as Campylobacter , in real-life processing conditions will make it possible to identify technological levers and better mitigate the microbial risk. • C. jejuni molecular response studied after consecutive thermal stresses by RT-qPCR. • Similar gene expression patterns shared between two of the three strains tested. • Up-regulated genes mainly involved in the heat shock response. • Down-regulated genes belonged to metabolic pathways (lipid, amino-acid metabolisms). • A strain-specific gene expression profile consistent with atypical stress response." @default.
• W3147639680 created "2021-04-13" @default.
• W3147639680 creator A5014451198 @default.
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• W3147639680 creator A5056076568 @default.
• W3147639680 creator A5062984156 @default.
• W3147639680 creator A5074296655 @default.
• W3147639680 date "2021-09-01" @default.
• W3147639680 modified "2023-09-27" @default.
• W3147639680 title "Quantification of Campylobacter jejuni gene expression after successive stresses mimicking poultry slaughtering steps" @default.
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