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- W3147657847 abstract "A endo-beta-glucanase Ⅱ （EGⅡ） cDNA gene was isolated from the fungus Humicola insolens H31-3 by RT-PCR, It was cloned into the expression vector pGAPZalphaA, and the resultant recombinant plasmid was introduced into Pichia pastoris GS115by electroporation after lineared by BspH Ⅰ digestion, The recombinant Pichia pastoris strain was obtained and SDS-PAGE showed that the apparent molecular weight of the expression product was about 55kD. The culture condition and the properties of the recombinant EGⅡ were characterized elementarily." @default.
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- W3147657847 date "2006-01-01" @default.
- W3147657847 modified "2023-09-24" @default.
- W3147657847 title "Cloning and expression of the endo--beta--glucanase II gene from Humicola insolens H31--3" @default.
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