Matches in SemOpenAlex for { <https://semopenalex.org/work/W3147678461> ?p ?o ?g. }
- W3147678461 endingPage "266" @default.
- W3147678461 startingPage "258" @default.
- W3147678461 abstract "To cryopreserve cells, it is essential to avoid intracellular ice formation during cooling and warming. One way to achieve this is to convert the water inside the cells into a non-crystalline glass. It is currently believed that to accomplish this vitrification, the cells must be suspended in a very high concentration (20-40%) of a glass-inducing solute, and subsequently cooled very rapidly. Herein, we report that this belief is erroneous with respect to the vitrification of one-cell rat embryos. In the present study, one-cell rat embryos were vitrified with 5 μL of EFS10 (a mixture of 10% ethylene glycol (EG), 27% Ficoll, and 0.45 M sucrose) in cryotubes at a moderate cooling rate, and warmed at various rates. Survival was assessed according to the ability of the cells to develop into blastocysts and to develop to term. When embryos were vitrified at a 2613 °C/min cooling rate and thawed by adding 1 mL of sucrose solution (0.3 M, 50 °C) at a warming rate of 18 467 °C/min, 58.1 ± 3.5% of the EFS10-vitrified embryos developed into blastocysts, and 50.0 ± 4.7% developed to term. These rates were similar to those of non-treated intact embryos. Using a conventional cryotube, we achieved developmental capabilities in one-cell rat embryos by rapid warming that were comparable to those of intact embryos, even using low concentrations (10%) of cell-permeating cryoprotectant and at low cooling rates." @default.
- W3147678461 created "2021-04-13" @default.
- W3147678461 creator A5001391751 @default.
- W3147678461 creator A5004036365 @default.
- W3147678461 creator A5011939127 @default.
- W3147678461 creator A5025816561 @default.
- W3147678461 creator A5025896052 @default.
- W3147678461 creator A5031661716 @default.
- W3147678461 creator A5038075616 @default.
- W3147678461 creator A5038468938 @default.
- W3147678461 creator A5052177979 @default.
- W3147678461 creator A5053222663 @default.
- W3147678461 creator A5069278443 @default.
- W3147678461 date "2021-03-30" @default.
- W3147678461 modified "2023-09-23" @default.
- W3147678461 title "Small-volume vitrification and rapid warming yield high survivals of one-cell rat embryos in cryotubes" @default.
- W3147678461 cites W1572476794 @default.
- W3147678461 cites W1963629394 @default.
- W3147678461 cites W1965764259 @default.
- W3147678461 cites W1974157915 @default.
- W3147678461 cites W1977387506 @default.
- W3147678461 cites W1983588838 @default.
- W3147678461 cites W1990787278 @default.
- W3147678461 cites W1997386840 @default.
- W3147678461 cites W1997519008 @default.
- W3147678461 cites W2009057295 @default.
- W3147678461 cites W2016202959 @default.
- W3147678461 cites W2032764699 @default.
- W3147678461 cites W2044159648 @default.
- W3147678461 cites W2061624989 @default.
- W3147678461 cites W2064297433 @default.
- W3147678461 cites W2066153852 @default.
- W3147678461 cites W2078602922 @default.
- W3147678461 cites W2093114783 @default.
- W3147678461 cites W2110462805 @default.
- W3147678461 cites W2112392835 @default.
- W3147678461 cites W2114413283 @default.
- W3147678461 cites W2138807866 @default.
- W3147678461 cites W2145780187 @default.
- W3147678461 cites W2162337503 @default.
- W3147678461 cites W2167492392 @default.
- W3147678461 cites W2168807103 @default.
- W3147678461 cites W2484900834 @default.
- W3147678461 cites W2740763407 @default.
- W3147678461 cites W2769767778 @default.
- W3147678461 cites W2790978766 @default.
- W3147678461 cites W2890663744 @default.
- W3147678461 cites W2942898131 @default.
- W3147678461 cites W3001434723 @default.
- W3147678461 cites W3046380072 @default.
- W3147678461 cites W2032620162 @default.
- W3147678461 doi "https://doi.org/10.1093/biolre/ioab059" @default.
- W3147678461 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/33783478" @default.
- W3147678461 hasPublicationYear "2021" @default.
- W3147678461 type Work @default.
- W3147678461 sameAs 3147678461 @default.
- W3147678461 citedByCount "2" @default.
- W3147678461 countsByYear W31476784612022 @default.
- W3147678461 crossrefType "journal-article" @default.
- W3147678461 hasAuthorship W3147678461A5001391751 @default.
- W3147678461 hasAuthorship W3147678461A5004036365 @default.
- W3147678461 hasAuthorship W3147678461A5011939127 @default.
- W3147678461 hasAuthorship W3147678461A5025816561 @default.
- W3147678461 hasAuthorship W3147678461A5025896052 @default.
- W3147678461 hasAuthorship W3147678461A5031661716 @default.
- W3147678461 hasAuthorship W3147678461A5038075616 @default.
- W3147678461 hasAuthorship W3147678461A5038468938 @default.
- W3147678461 hasAuthorship W3147678461A5052177979 @default.
- W3147678461 hasAuthorship W3147678461A5053222663 @default.
- W3147678461 hasAuthorship W3147678461A5069278443 @default.
- W3147678461 hasConcept C112964491 @default.
- W3147678461 hasConcept C121332964 @default.
- W3147678461 hasConcept C137061746 @default.
- W3147678461 hasConcept C16685009 @default.
- W3147678461 hasConcept C178790620 @default.
- W3147678461 hasConcept C179933525 @default.
- W3147678461 hasConcept C185592680 @default.
- W3147678461 hasConcept C196843134 @default.
- W3147678461 hasConcept C202751555 @default.
- W3147678461 hasConcept C2777516009 @default.
- W3147678461 hasConcept C2778022349 @default.
- W3147678461 hasConcept C2778597767 @default.
- W3147678461 hasConcept C5362183 @default.
- W3147678461 hasConcept C55493867 @default.
- W3147678461 hasConcept C68528507 @default.
- W3147678461 hasConcept C71924100 @default.
- W3147678461 hasConcept C86803240 @default.
- W3147678461 hasConcept C95053279 @default.
- W3147678461 hasConcept C95444343 @default.
- W3147678461 hasConcept C97355855 @default.
- W3147678461 hasConceptScore W3147678461C112964491 @default.
- W3147678461 hasConceptScore W3147678461C121332964 @default.
- W3147678461 hasConceptScore W3147678461C137061746 @default.
- W3147678461 hasConceptScore W3147678461C16685009 @default.
- W3147678461 hasConceptScore W3147678461C178790620 @default.
- W3147678461 hasConceptScore W3147678461C179933525 @default.
- W3147678461 hasConceptScore W3147678461C185592680 @default.
- W3147678461 hasConceptScore W3147678461C196843134 @default.