Matches in SemOpenAlex for { <https://semopenalex.org/work/W3147746504> ?p ?o ?g. }
Showing items 1 to 97 of
97
with 100 items per page.
- W3147746504 endingPage "5" @default.
- W3147746504 startingPage "1" @default.
- W3147746504 abstract "The 5’-thiolated DNA probe of Meq oncogenic gene consisting of 24 deoxynucleotide, was reduced by Dithiothreitol (DTT) and immobilized covalently via thiolated bond onto gold electrode followed by hybridization with 1-4 ng/μl of Marek’s Disease Virus (MDV) Single-Stranded (ss) DNA probe from tumor sample of chicken for 10 min at room temp. The Meqgenosensor could detect 1 ngss-DNA in tumor samples in min by Cyclic Voltammetry (CV), Differential Pulse Voltammetry (DPV) and Electrochemical Impedance (EI). The ssDNA modified Au electrode was characterized by Fourier Transform Infrared Spectroscopy (FTIR) and Scanning Electron Microscopy (SEM). An amperometric genosensor was constructed for detection of Meq gene by connecting ssDNA electrode modified Au electrode (working electrode) with Ag/ AgCl electrode (reference electrode) and Pt wire (as auxiliary electrode) through potentiostat. The hybridization between ssDNA probe and target ssDNA was detected by reduction in current generated by interaction of Methylene Blue (MB) with free G’ of single stranded oligonucleotide. The ssDNA Au electrode showed optimum current within 10 min at pH8, 250C. This is the first report on construction of genosensor for the simple, fast and specific detection of MDV in chickens." @default.
- W3147746504 created "2021-04-13" @default.
- W3147746504 creator A5000437044 @default.
- W3147746504 creator A5011132895 @default.
- W3147746504 creator A5021712020 @default.
- W3147746504 creator A5041219986 @default.
- W3147746504 creator A5056385123 @default.
- W3147746504 creator A5067783117 @default.
- W3147746504 creator A5072329703 @default.
- W3147746504 date "2021-03-23" @default.
- W3147746504 modified "2023-09-24" @default.
- W3147746504 title "An Amperometric Genosensor for Detection of Virus Causing Marek’s Disease in Poultry" @default.
- W3147746504 hasPublicationYear "2021" @default.
- W3147746504 type Work @default.
- W3147746504 sameAs 3147746504 @default.
- W3147746504 citedByCount "0" @default.
- W3147746504 crossrefType "journal-article" @default.
- W3147746504 hasAuthorship W3147746504A5000437044 @default.
- W3147746504 hasAuthorship W3147746504A5011132895 @default.
- W3147746504 hasAuthorship W3147746504A5021712020 @default.
- W3147746504 hasAuthorship W3147746504A5041219986 @default.
- W3147746504 hasAuthorship W3147746504A5056385123 @default.
- W3147746504 hasAuthorship W3147746504A5067783117 @default.
- W3147746504 hasAuthorship W3147746504A5072329703 @default.
- W3147746504 hasConcept C113196181 @default.
- W3147746504 hasConcept C119128265 @default.
- W3147746504 hasConcept C121332964 @default.
- W3147746504 hasConcept C129312508 @default.
- W3147746504 hasConcept C130197536 @default.
- W3147746504 hasConcept C139369640 @default.
- W3147746504 hasConcept C147789679 @default.
- W3147746504 hasConcept C160892712 @default.
- W3147746504 hasConcept C17525397 @default.
- W3147746504 hasConcept C185592680 @default.
- W3147746504 hasConcept C192562407 @default.
- W3147746504 hasConcept C2777869211 @default.
- W3147746504 hasConcept C2779525745 @default.
- W3147746504 hasConcept C2779995598 @default.
- W3147746504 hasConcept C43617362 @default.
- W3147746504 hasConcept C52859227 @default.
- W3147746504 hasConcept C552990157 @default.
- W3147746504 hasConcept C55493867 @default.
- W3147746504 hasConcept C62520636 @default.
- W3147746504 hasConcept C69118416 @default.
- W3147746504 hasConcept C7040849 @default.
- W3147746504 hasConceptScore W3147746504C113196181 @default.
- W3147746504 hasConceptScore W3147746504C119128265 @default.
- W3147746504 hasConceptScore W3147746504C121332964 @default.
- W3147746504 hasConceptScore W3147746504C129312508 @default.
- W3147746504 hasConceptScore W3147746504C130197536 @default.
- W3147746504 hasConceptScore W3147746504C139369640 @default.
- W3147746504 hasConceptScore W3147746504C147789679 @default.
- W3147746504 hasConceptScore W3147746504C160892712 @default.
- W3147746504 hasConceptScore W3147746504C17525397 @default.
- W3147746504 hasConceptScore W3147746504C185592680 @default.
- W3147746504 hasConceptScore W3147746504C192562407 @default.
- W3147746504 hasConceptScore W3147746504C2777869211 @default.
- W3147746504 hasConceptScore W3147746504C2779525745 @default.
- W3147746504 hasConceptScore W3147746504C2779995598 @default.
- W3147746504 hasConceptScore W3147746504C43617362 @default.
- W3147746504 hasConceptScore W3147746504C52859227 @default.
- W3147746504 hasConceptScore W3147746504C552990157 @default.
- W3147746504 hasConceptScore W3147746504C55493867 @default.
- W3147746504 hasConceptScore W3147746504C62520636 @default.
- W3147746504 hasConceptScore W3147746504C69118416 @default.
- W3147746504 hasConceptScore W3147746504C7040849 @default.
- W3147746504 hasIssue "3" @default.
- W3147746504 hasLocation W31477465041 @default.
- W3147746504 hasOpenAccess W3147746504 @default.
- W3147746504 hasPrimaryLocation W31477465041 @default.
- W3147746504 hasRelatedWork W1991978520 @default.
- W3147746504 hasRelatedWork W1995470353 @default.
- W3147746504 hasRelatedWork W1996439655 @default.
- W3147746504 hasRelatedWork W2005020734 @default.
- W3147746504 hasRelatedWork W2010778053 @default.
- W3147746504 hasRelatedWork W2015964925 @default.
- W3147746504 hasRelatedWork W2047515423 @default.
- W3147746504 hasRelatedWork W2076280211 @default.
- W3147746504 hasRelatedWork W2082447740 @default.
- W3147746504 hasRelatedWork W2082983148 @default.
- W3147746504 hasRelatedWork W2132371057 @default.
- W3147746504 hasRelatedWork W2158129080 @default.
- W3147746504 hasRelatedWork W2173676312 @default.
- W3147746504 hasRelatedWork W2255367931 @default.
- W3147746504 hasRelatedWork W2358427043 @default.
- W3147746504 hasRelatedWork W2367657260 @default.
- W3147746504 hasRelatedWork W2475052885 @default.
- W3147746504 hasRelatedWork W2772290488 @default.
- W3147746504 hasRelatedWork W2792352720 @default.
- W3147746504 hasRelatedWork W3169355769 @default.
- W3147746504 hasVolume "2021" @default.
- W3147746504 isParatext "false" @default.
- W3147746504 isRetracted "false" @default.
- W3147746504 magId "3147746504" @default.
- W3147746504 workType "article" @default.