Matches in SemOpenAlex for { <https://semopenalex.org/work/W3147821205> ?p ?o ?g. }
Showing items 1 to 80 of
80
with 100 items per page.
- W3147821205 abstract "As part of the innate immune system, the important cellular pattern recognition receptor (PRR) RIG-I senses the presence of incoming viral particles by binding viral RNA, leading to the transcription of type I interferons (IFN). These cytokines are then secreted and bind to the type I IFN receptor (IFNAR), which induces expression of hundreds of interferon-stimulated gene products (ISGs) that generate an antiviral environment. Kaposi’s sarcoma-associated herpesvirus (KSHV) is a human oncovirus. To establish lifelong infection, KSHV has evolved sophisticated mechanisms to modulate the innate immune response of its host. We identified the poorly characterised KSHV ORF20 protein as a potent inhibitor of the RIG-I-mediated type I IFN response using a luciferase-based reporter screen with 85 open reading frames (ORFs). To better understand the role of ORF20, we used the unbiased approach of quantitative affinity purification coupled to mass spectrometry (q-AP-MS) to identify the cellular interaction partners of ORF20. We found that ORF20 interacted with a variety of ribosomal and nucleolar proteins. Immunofluorescence analysis showed their colocalisation in the nucleolus. Interestingly, distinct localisation patterns of ORF20 correlated with the dispersal of nucleolar and ribosomal proteins from the nucleolus. Furthermore, the antiviral ISG oligoadenylate synthetase-like protein (OASL) was identified as an interaction partner of ORF20 by q-AP-MS. We found that ORF20 specifically interacted with the OAS domain of OASL and that ORF20 and OASL colocalised in the nucleolus. OASL was shown to amplify the type I IFN activation by directly interacting with RIG-I. However, we found that ORF20 did not interfere with colocalisation or interaction of OASL and RIG-I. Surprisingly, we found that the inhibitory effect of ORF20 on RIG-I signalling was independent of OASL expression. By analysing the cellular interaction partners of OASL by q-AP-MS, we found that ORF20 and OASL shared a highly similar interactome. Like ORF20, OASL interacted and colocalised with numerous ribosomal and nucleolar proteins. As the nucleolus is the site of ribosome biogenesis and ORF20 and OASL interact with a number of ribosomal proteins, our data suggest that the cellular protein OASL may have a role for ribosome function, which might be manipulated by the KSHV protein ORF20 to promote KSHV infection." @default.
- W3147821205 created "2021-04-13" @default.
- W3147821205 creator A5070756337 @default.
- W3147821205 date "2019-03-04" @default.
- W3147821205 modified "2023-09-24" @default.
- W3147821205 title "Characterisation of the KSHV protein ORF20 and its role in innate immunity" @default.
- W3147821205 doi "https://doi.org/10.24355/dbbs.084-201903041425-0" @default.
- W3147821205 hasPublicationYear "2019" @default.
- W3147821205 type Work @default.
- W3147821205 sameAs 3147821205 @default.
- W3147821205 citedByCount "2" @default.
- W3147821205 countsByYear W31478212052020 @default.
- W3147821205 countsByYear W31478212052021 @default.
- W3147821205 crossrefType "dissertation" @default.
- W3147821205 hasAuthorship W3147821205A5070756337 @default.
- W3147821205 hasConcept C104317684 @default.
- W3147821205 hasConcept C111289621 @default.
- W3147821205 hasConcept C136449434 @default.
- W3147821205 hasConcept C159047783 @default.
- W3147821205 hasConcept C170493617 @default.
- W3147821205 hasConcept C183873130 @default.
- W3147821205 hasConcept C190062978 @default.
- W3147821205 hasConcept C2522874641 @default.
- W3147821205 hasConcept C2776178377 @default.
- W3147821205 hasConcept C2776409557 @default.
- W3147821205 hasConcept C2776954689 @default.
- W3147821205 hasConcept C2780727368 @default.
- W3147821205 hasConcept C2780797929 @default.
- W3147821205 hasConcept C38062823 @default.
- W3147821205 hasConcept C54355233 @default.
- W3147821205 hasConcept C67705224 @default.
- W3147821205 hasConcept C86803240 @default.
- W3147821205 hasConcept C88478588 @default.
- W3147821205 hasConcept C95444343 @default.
- W3147821205 hasConceptScore W3147821205C104317684 @default.
- W3147821205 hasConceptScore W3147821205C111289621 @default.
- W3147821205 hasConceptScore W3147821205C136449434 @default.
- W3147821205 hasConceptScore W3147821205C159047783 @default.
- W3147821205 hasConceptScore W3147821205C170493617 @default.
- W3147821205 hasConceptScore W3147821205C183873130 @default.
- W3147821205 hasConceptScore W3147821205C190062978 @default.
- W3147821205 hasConceptScore W3147821205C2522874641 @default.
- W3147821205 hasConceptScore W3147821205C2776178377 @default.
- W3147821205 hasConceptScore W3147821205C2776409557 @default.
- W3147821205 hasConceptScore W3147821205C2776954689 @default.
- W3147821205 hasConceptScore W3147821205C2780727368 @default.
- W3147821205 hasConceptScore W3147821205C2780797929 @default.
- W3147821205 hasConceptScore W3147821205C38062823 @default.
- W3147821205 hasConceptScore W3147821205C54355233 @default.
- W3147821205 hasConceptScore W3147821205C67705224 @default.
- W3147821205 hasConceptScore W3147821205C86803240 @default.
- W3147821205 hasConceptScore W3147821205C88478588 @default.
- W3147821205 hasConceptScore W3147821205C95444343 @default.
- W3147821205 hasLocation W31478212051 @default.
- W3147821205 hasOpenAccess W3147821205 @default.
- W3147821205 hasPrimaryLocation W31478212051 @default.
- W3147821205 hasRelatedWork W1966380622 @default.
- W3147821205 hasRelatedWork W1973907427 @default.
- W3147821205 hasRelatedWork W2005496691 @default.
- W3147821205 hasRelatedWork W2084761561 @default.
- W3147821205 hasRelatedWork W2094927612 @default.
- W3147821205 hasRelatedWork W2112561439 @default.
- W3147821205 hasRelatedWork W2136686995 @default.
- W3147821205 hasRelatedWork W2158692057 @default.
- W3147821205 hasRelatedWork W2345822128 @default.
- W3147821205 hasRelatedWork W2403028678 @default.
- W3147821205 hasRelatedWork W2548576515 @default.
- W3147821205 hasRelatedWork W2554669965 @default.
- W3147821205 hasRelatedWork W2922231529 @default.
- W3147821205 hasRelatedWork W2941629117 @default.
- W3147821205 hasRelatedWork W2965819944 @default.
- W3147821205 hasRelatedWork W2978959465 @default.
- W3147821205 hasRelatedWork W3091823762 @default.
- W3147821205 hasRelatedWork W3129357011 @default.
- W3147821205 hasRelatedWork W3166963288 @default.
- W3147821205 hasRelatedWork W3201520141 @default.
- W3147821205 isParatext "false" @default.
- W3147821205 isRetracted "false" @default.
- W3147821205 magId "3147821205" @default.
- W3147821205 workType "dissertation" @default.