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- W3147905929 abstract "Objective:To observe the effect of Kupfler cells on immune tolerance in liver transplantation. Methods:The rats were randomly divided into A,B and C groups.A group was sham operation group.The donor rats of group B had intraperitoneal injection of 1 nmol Kuppffer cells every other day for three days before liver transplantation.Rats of group C were injected with equal saline.The rat liver transplantation models were established by modified Kamada’s two-cuff technique.The rats were sacrificed after 24 hours.The concentrations of ALT and AST in serum were measured with the biochemical analyzer.The level of IL-2 and TNF- a in serum were measured by ELISA method.The apoptotic indexes were detected by immunohistochemical assay. Results:The concentration of ALT,AST,IL-1 and TNF- a in A,B and C groups were increased successively.The levels of group C were significantly higher than that of group B and A(P<0.05), and the levels of group B were significantly higher than that of group A(P<0.05).The apoptotic indexes of three groups were 3.40±0.37,14.70±2.54 and 26.33±3.65,respectively,with significant difference among three groups(P<0.05).Conclusions:Pretreatment with Kupfler cells can reduce liver injury and raise liver transplantation immune tolerance." @default.
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- W3147905929 date "2012-01-01" @default.
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- W3147905929 title "Effect of Kupffer cells on immune tolerance in liver transplantation" @default.
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