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- W3148093722 abstract "Ethnopharmacological relevance: Flower bud of Tussilago farfara L. is widely used for the treatment of cough, bronchitis and asthmatic disorders in the Traditional Chinese Medicine. However, due to the increasing demands, adulteration with rachis is frequently encountered in the marketplace. No report demonstrated the chemical and pharmacological differences between flower bud and rachis before. Materials and methods: The water extracts were orally administrated to mice. Ammonia induced mice coughing model was used to evaluate the antitussive activity. The expectorant activity was evaluated by volume of phenol red in mice’s tracheas. Metabolites were identified directly from the crude extracts through 1Dand 2D-NMR spectra. A metabolic profiling carried out by 1H NMR spectroscopy and multivariate data analysis was applied to crude extracts from flower bud and rachis. Results: Flower bud significantly lengthened the latent period of cough, decreased cough frequency caused by ammonia and enhanced tracheal phenol red output in expectorant evaluation. Principal component analysis (PCA) yielded good separation between flower bud and rachis, and corresponding loading plot showed that the phenolic compounds, organic acid, sugar, amino acid, terpene and sterol contributed to" @default.
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- W3148093722 date "2012-01-01" @default.
- W3148093722 modified "2023-09-26" @default.
- W3148093722 title "Metabolomic profiling of the flower bud and rachis of Tussilago farfara with antitussive and expectorant effects on mice" @default.
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