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- W3148109044 abstract "Objective To establish a rapid and convenient method of reverse dot blot (RDB) analysis for detecting the point mutations of non-deletion α-thalassemia in Chinese. Methods Label biotin to primers and amplify human α2 globin gene selectively, then hybridize PCR products with a set of oligonucleotide probes immobilized on strips, and develop colour to detect non-deletion α-thalassemia defects. Results The PCR system using biotin-labeled primers could specifically amplify a 1085 bp fragment of human α2 globin gene which encompasses all six α-thalassemia mutations. After being hybridized with sequence-specific oligonucleotide probes and colour development, it could simultaneously identify all six types of non-deletion α-thalassemias encountered in Chinese. Conclusion This method does not need semi-nested PCR, and the products amplified by biotinylated primers can be used directly to hybridize with the probes on strips under the identical conditions of hybridization. So, it is a specific and multiplex detection assay for screening non-deletion α-thalassemia defects in Chinese." @default.
- W3148109044 created "2021-04-13" @default.
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- W3148109044 date "2003-01-01" @default.
- W3148109044 modified "2023-09-23" @default.
- W3148109044 title "Rapid diagnosis of non-deletion α-thalassemias by reverse dot blot" @default.
- W3148109044 hasPublicationYear "2003" @default.
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