Matches in SemOpenAlex for { <https://semopenalex.org/work/W3148194714> ?p ?o ?g. }
Showing items 1 to 84 of
84
with 100 items per page.
- W3148194714 endingPage "95" @default.
- W3148194714 startingPage "92" @default.
- W3148194714 abstract "Summary: To compare the anti-tumor effects of transmembrane TNF-α (TM-TNF) and secreted TNF-α (S-TNF) in vivo, mouse fibroblasts NIH3T3 were transfected separately with three types of retrovirus containing wild type TNF-α (Wt-TNF), TM-TNF mutant (TM-TNFm), S-TNF mu tant (S-TNFm). Southern blot, RT-PCR, FACS and bioassay were used to investigate TNF-α gene integration, expression and its biological activity. It was found that both fixed cells and supernatant of NIH3T3/Wt-TNF, the fixed cells of NIH3T3/TM-TNFm and the supernatant of NIH3T3/S-TNFm could express high level of TNF-α or its mutants and effectively kill H22 in vitro. The trans fected NIH3T3 were separately injected into the mice at the sites of H22 tumor cell inoculation ac cording to a ratio of 5: 1 or 1: 1 (effector/target cells, E/T) after the third day of H22 challenge,respectively. At the E/T= 5 : 1, the NIH3T3/TM-TNFm induced the highest tumor regression,while NIH3T3/S-TNFm exerted the strongest tumor depressing effect at the E/T= 1 .: 1 in vivo. No obvious side effects were noted throughout the course of treatment. The results suggest that both TM-TNF and S-TNF could cause tumor regression. The anti-tumor effect of TM-TNF would be more powerful and safe than that of S-TNF at the proper E/T ratio." @default.
- W3148194714 created "2021-04-13" @default.
- W3148194714 creator A5000169719 @default.
- W3148194714 creator A5028256429 @default.
- W3148194714 creator A5032420603 @default.
- W3148194714 creator A5053780153 @default.
- W3148194714 creator A5066180979 @default.
- W3148194714 creator A5074164414 @default.
- W3148194714 creator A5081652936 @default.
- W3148194714 date "2002-01-01" @default.
- W3148194714 modified "2023-09-26" @default.
- W3148194714 title "Antitumor Effects of the Fibroblasts Transfected TNF-α Gene and its Mutants" @default.
- W3148194714 hasPublicationYear "2002" @default.
- W3148194714 type Work @default.
- W3148194714 sameAs 3148194714 @default.
- W3148194714 citedByCount "0" @default.
- W3148194714 crossrefType "journal-article" @default.
- W3148194714 hasAuthorship W3148194714A5000169719 @default.
- W3148194714 hasAuthorship W3148194714A5028256429 @default.
- W3148194714 hasAuthorship W3148194714A5032420603 @default.
- W3148194714 hasAuthorship W3148194714A5053780153 @default.
- W3148194714 hasAuthorship W3148194714A5066180979 @default.
- W3148194714 hasAuthorship W3148194714A5074164414 @default.
- W3148194714 hasAuthorship W3148194714A5081652936 @default.
- W3148194714 hasConcept C104317684 @default.
- W3148194714 hasConcept C104488531 @default.
- W3148194714 hasConcept C143065580 @default.
- W3148194714 hasConcept C153911025 @default.
- W3148194714 hasConcept C17991360 @default.
- W3148194714 hasConcept C185592680 @default.
- W3148194714 hasConcept C202751555 @default.
- W3148194714 hasConcept C203014093 @default.
- W3148194714 hasConcept C207001950 @default.
- W3148194714 hasConcept C2776415932 @default.
- W3148194714 hasConcept C51785407 @default.
- W3148194714 hasConcept C54009773 @default.
- W3148194714 hasConcept C54355233 @default.
- W3148194714 hasConcept C55493867 @default.
- W3148194714 hasConcept C86803240 @default.
- W3148194714 hasConceptScore W3148194714C104317684 @default.
- W3148194714 hasConceptScore W3148194714C104488531 @default.
- W3148194714 hasConceptScore W3148194714C143065580 @default.
- W3148194714 hasConceptScore W3148194714C153911025 @default.
- W3148194714 hasConceptScore W3148194714C17991360 @default.
- W3148194714 hasConceptScore W3148194714C185592680 @default.
- W3148194714 hasConceptScore W3148194714C202751555 @default.
- W3148194714 hasConceptScore W3148194714C203014093 @default.
- W3148194714 hasConceptScore W3148194714C207001950 @default.
- W3148194714 hasConceptScore W3148194714C2776415932 @default.
- W3148194714 hasConceptScore W3148194714C51785407 @default.
- W3148194714 hasConceptScore W3148194714C54009773 @default.
- W3148194714 hasConceptScore W3148194714C54355233 @default.
- W3148194714 hasConceptScore W3148194714C55493867 @default.
- W3148194714 hasConceptScore W3148194714C86803240 @default.
- W3148194714 hasIssue "2" @default.
- W3148194714 hasLocation W31481947141 @default.
- W3148194714 hasOpenAccess W3148194714 @default.
- W3148194714 hasPrimaryLocation W31481947141 @default.
- W3148194714 hasRelatedWork W1481938358 @default.
- W3148194714 hasRelatedWork W1611738264 @default.
- W3148194714 hasRelatedWork W2027655179 @default.
- W3148194714 hasRelatedWork W2062530388 @default.
- W3148194714 hasRelatedWork W2100273527 @default.
- W3148194714 hasRelatedWork W2122913844 @default.
- W3148194714 hasRelatedWork W2135596975 @default.
- W3148194714 hasRelatedWork W2141001643 @default.
- W3148194714 hasRelatedWork W2162465275 @default.
- W3148194714 hasRelatedWork W2350878818 @default.
- W3148194714 hasRelatedWork W2370834633 @default.
- W3148194714 hasRelatedWork W2379463607 @default.
- W3148194714 hasRelatedWork W2380263979 @default.
- W3148194714 hasRelatedWork W2394192597 @default.
- W3148194714 hasRelatedWork W2394268564 @default.
- W3148194714 hasRelatedWork W2419927842 @default.
- W3148194714 hasRelatedWork W2889145805 @default.
- W3148194714 hasRelatedWork W3134829704 @default.
- W3148194714 hasRelatedWork W3148190780 @default.
- W3148194714 hasRelatedWork W3030675128 @default.
- W3148194714 isParatext "false" @default.
- W3148194714 isRetracted "false" @default.
- W3148194714 magId "3148194714" @default.
- W3148194714 workType "article" @default.