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- W3148229311 abstract "Objective To explore the inhibition effects of 10-23 DNAzymes with different substrate-recognition domains targeting hepatitis B virus (HBV) S gene and C gene expression in 2.2.15 cells. Methods 10-23 DNAzymes with different substrate-recognition domains specific to HBV S gene open reading frame (ORF) A 157UG and HBV C gene ORF A 1816UG were designed and synthesized, respectively. Different 10-23 DNAzymes were transfected into 2.2.15 cells which is a stable HBV producing cell line. HBsAg and HBeAg secreted into culture media were detected by radioimmunoassay (RIA) and HBV DNA levels were measured by real-time PCR. 3-(4,5-dimethylthiagol-2-yl)-2,5-drphnyl tetragolium brornide (MTT) assays were performed to evaluate cytotoxicity. Results HBsAg and HBeAg expressions were reduced by various DNAzymes (0.1-2.5 μmol/L) with different substrate-recognition domains after transfection. The antiviral effects of DNAzymes were apparent until 72 h post-transfection. The inhibition rates of the DNAzymes at the same dose on HBsAg and HBeAg in the same period of post-transfection were as the following: DrzBS-9DrzBS-8DrzBS-7; DrzBC-9DrzBC-8DrzBC-7. Among all the DNAzymes used, DrzBS-9 targeting S gene and DrzBC-9 targeting C gene were most potent, with HBsAg and HBeAg reduced 95% and 92% 48 h post-transfection at the dose of 2.5 μmol/L, respectively. The inhibition effects on HBV DNA by various DNAzymes with different substrate-recognition domains were of no significance. There were no evident cytotoxic effects of these DNAzymes in the range from 0.1 to 2.5 μmol/L. Conclusion 10-23 DNAzymes with different substrate-recognition domains targeting HBV S gene and C gene mRNA possessed specific inhibition effects in 2.2.15 cells, and DrzBS-9 targeting S gene and DrzBC-9 targeting C gene were most potent." @default.
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- W3148229311 date "2006-01-01" @default.
- W3148229311 modified "2023-09-24" @default.
- W3148229311 title "Inhibition of hepatitis B virus S gene and C gene expression by different 10-23 DNAzymes substrate-recognition domains" @default.
- W3148229311 hasPublicationYear "2006" @default.
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