FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W3148369966> ?p ?o ?g. }

• W3148369966 abstract "Abstract Membrane-less organelles (MLOs) formed by liquid-liquid phase separation (LLPS) play pivotal roles in biological processes. During LLPS, proteins and nucleotides are extremely condensed, resulting in changes of their conformation and biological functions. Disturbed LLPS homeostasis in MLOs cause fatal diseases such as amyotrophic lateral sclerosis. Therefore, it is important to detect changes of the degree of crowding in MLOs. However, it has not been investigated well due to lack of an appropriate method. To address this, we developed a genetically-encoded molecular crowding sensor CRONOS that senses the degree of macromolecular crowding in MLOs using fluorescence resonance energy transfer (FRET) system. CRONOS is a very bright biosensor with wider dynamic range and detect changes in the macromolecular volume fraction better than the previously reported mCer-mCit sensor in solution. By fusing to scaffold protein of each MLO, we successfully delivered CRONOS to MLO of interest and detected previously undescribed difference of the degree of crowding in each MLO. If not tagged, CRONOS localized to interstitial space of MLOs, giving us the crowding information of inspace. CRONOS also detected changes of degree of macromolecular crowding in nucleolus induced by environmental stress or inhibition of transcription. These findings suggest that CRONOS can be a useful tool for determination of molecular crowding and detection of pathological changes in MLOs in live cells." @default.
• W3148369966 created "2021-04-13" @default.
• W3148369966 creator A5002554636 @default.
• W3148369966 creator A5005230545 @default.
• W3148369966 creator A5040905965 @default.
• W3148369966 creator A5048501201 @default.
• W3148369966 creator A5050148199 @default.
• W3148369966 creator A5064267364 @default.
• W3148369966 creator A5065667748 @default.
• W3148369966 date "2021-04-01" @default.
• W3148369966 modified "2023-10-16" @default.
• W3148369966 title "An improved molecular crowding sensor CRONOS for detection of crowding changes in membrane-less organelles under pathological conditions" @default.
• W3148369966 cites W1504137373 @default.
• W3148369966 cites W1529084957 @default.
• W3148369966 cites W2002373110 @default.
• W3148369966 cites W2017288508 @default.
• W3148369966 cites W2022889543 @default.
• W3148369966 cites W2034728705 @default.
• W3148369966 cites W2054543531 @default.
• W3148369966 cites W2077502289 @default.
• W3148369966 cites W2150766477 @default.
• W3148369966 cites W2295382293 @default.
• W3148369966 cites W2410250138 @default.
• W3148369966 cites W2533664860 @default.
• W3148369966 cites W2551836638 @default.
• W3148369966 cites W2595869676 @default.
• W3148369966 cites W2612757501 @default.
• W3148369966 cites W2753162941 @default.
• W3148369966 cites W2790446628 @default.
• W3148369966 cites W2888163308 @default.
• W3148369966 cites W2898086190 @default.
• W3148369966 cites W2901094661 @default.
• W3148369966 cites W2905220927 @default.
• W3148369966 cites W2914907359 @default.
• W3148369966 cites W2916646997 @default.
• W3148369966 cites W2924556456 @default.
• W3148369966 cites W2954571074 @default.
• W3148369966 cites W2956385297 @default.
• W3148369966 cites W2977383991 @default.
• W3148369966 cites W3005496808 @default.
• W3148369966 cites W3025909849 @default.
• W3148369966 cites W3034018673 @default.
• W3148369966 cites W3101848056 @default.
• W3148369966 doi "https://doi.org/10.1101/2021.03.31.437991" @default.
• W3148369966 hasPublicationYear "2021" @default.
• W3148369966 type Work @default.
• W3148369966 sameAs 3148369966 @default.
• W3148369966 citedByCount "2" @default.
• W3148369966 countsByYear W31483699662021 @default.
• W3148369966 crossrefType "posted-content" @default.
• W3148369966 hasAuthorship W3148369966A5002554636 @default.
• W3148369966 hasAuthorship W3148369966A5005230545 @default.
• W3148369966 hasAuthorship W3148369966A5040905965 @default.
• W3148369966 hasAuthorship W3148369966A5048501201 @default.
• W3148369966 hasAuthorship W3148369966A5050148199 @default.
• W3148369966 hasAuthorship W3148369966A5064267364 @default.
• W3148369966 hasAuthorship W3148369966A5065667748 @default.
• W3148369966 hasBestOaLocation W31483699661 @default.
• W3148369966 hasConcept C121332964 @default.
• W3148369966 hasConcept C12554922 @default.
• W3148369966 hasConcept C149333683 @default.
• W3148369966 hasConcept C168240541 @default.
• W3148369966 hasConcept C169760540 @default.
• W3148369966 hasConcept C181990884 @default.
• W3148369966 hasConcept C204389451 @default.
• W3148369966 hasConcept C55493867 @default.
• W3148369966 hasConcept C62520636 @default.
• W3148369966 hasConcept C86803240 @default.
• W3148369966 hasConcept C91881484 @default.
• W3148369966 hasConcept C95444343 @default.
• W3148369966 hasConcept C96305047 @default.
• W3148369966 hasConceptScore W3148369966C121332964 @default.
• W3148369966 hasConceptScore W3148369966C12554922 @default.
• W3148369966 hasConceptScore W3148369966C149333683 @default.
• W3148369966 hasConceptScore W3148369966C168240541 @default.
• W3148369966 hasConceptScore W3148369966C169760540 @default.
• W3148369966 hasConceptScore W3148369966C181990884 @default.
• W3148369966 hasConceptScore W3148369966C204389451 @default.
• W3148369966 hasConceptScore W3148369966C55493867 @default.
• W3148369966 hasConceptScore W3148369966C62520636 @default.
• W3148369966 hasConceptScore W3148369966C86803240 @default.
• W3148369966 hasConceptScore W3148369966C91881484 @default.
• W3148369966 hasConceptScore W3148369966C95444343 @default.
• W3148369966 hasConceptScore W3148369966C96305047 @default.
• W3148369966 hasLocation W31483699661 @default.
• W3148369966 hasOpenAccess W3148369966 @default.
• W3148369966 hasPrimaryLocation W31483699661 @default.
• W3148369966 hasRelatedWork W1968689021 @default.
• W3148369966 hasRelatedWork W2005892731 @default.
• W3148369966 hasRelatedWork W2054543531 @default.
• W3148369966 hasRelatedWork W2062595694 @default.
• W3148369966 hasRelatedWork W2788565759 @default.
• W3148369966 hasRelatedWork W2989223770 @default.
• W3148369966 hasRelatedWork W3043189512 @default.
• W3148369966 hasRelatedWork W3148369966 @default.
• W3148369966 hasRelatedWork W3211237591 @default.
• W3148369966 hasRelatedWork W4362692235 @default.
• W3148369966 isParatext "false" @default.
• W3148369966 isRetracted "false" @default.
• W3148369966 magId "3148369966" @default.

• next