FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W3148392242> ?p ?o ?g. }

• W3148392242 abstract "MicroRNA (miR)-19b is part of the miR-17-92 cluster associated with cardiac development. Here, we investigated the effects of overexpressing miR-19b on proliferation, differentiation, apoptosis, and regulation of the Wnt/b-catenin signaling pathway in the multipotent murine P19 cell line that can be induced to undergo car- diogenesis. P19 cells were transfected with the miR-19b plasmid or empty vector, and miR-19b overexpression was verified by Quantitative Real-Time PCR (qPCR). The miR- 19b or vector control stable cell lines were selected using Blasticidin S HCl, and their proliferation, cell cycle, and apoptosis levels were analyzed using the Cell Counting Kit-8 and flow cytometry. P19 cell differentiation markers, apoptosis-related genes (bax, bcl-2), and Wnt/b-catenin signaling pathway-related genes were detected by qPCR, the corresponding proteins by Western blot. Expression of the Wnt pathway and differentiation marker proteins was also verified by immunofluorescence. Morphological changes associated with apoptosis were observed by elec- tron microscopy and Hoechst staining. On the basis of these results, we demonstrated that miR-19b overexpres- sion promoted proliferation and differentiation but inhib- ited apoptosis in P19 cells; Wnt and b-catenin expressions were decreased, while that of GSK3b was increased with miR-19b overexpression. Overexpression of miR-19b inhibited activation of the Wnt/b-catenin signaling path- way in P19 cells, which may regulate cardiomyocyte dif- ferentiation. Our findings may bring new insights into the mechanisms underlying cardiac diseases and suggest that miR-19b is a potential new therapeutic target for cardio- vascular diseases." @default.
• W3148392242 created "2021-04-13" @default.
• W3148392242 creator A5005983180 @default.
• W3148392242 creator A5079319835 @default.
• W3148392242 date "2013-01-01" @default.
• W3148392242 modified "2023-09-27" @default.
• W3148392242 title "Effects of miR-19b Overexpression on Proliferation, Differentiation, Apoptosis and Wnt/b-Catenin Signaling Pathway in P19 Cell Model of Cardiac Differentiation In Vitro" @default.
• W3148392242 cites W1967103142 @default.
• W3148392242 cites W1973278012 @default.
• W3148392242 cites W1990568577 @default.
• W3148392242 cites W2000043946 @default.
• W3148392242 cites W2004588189 @default.
• W3148392242 cites W2005488930 @default.
• W3148392242 cites W2006743934 @default.
• W3148392242 cites W2009501454 @default.
• W3148392242 cites W2011810771 @default.
• W3148392242 cites W2012517517 @default.
• W3148392242 cites W2014946489 @default.
• W3148392242 cites W2016823790 @default.
• W3148392242 cites W2023121881 @default.
• W3148392242 cites W2026570544 @default.
• W3148392242 cites W2030946712 @default.
• W3148392242 cites W2036676672 @default.
• W3148392242 cites W2039473936 @default.
• W3148392242 cites W2042748226 @default.
• W3148392242 cites W2042773729 @default.
• W3148392242 cites W2060515857 @default.
• W3148392242 cites W2068637372 @default.
• W3148392242 cites W2075477970 @default.
• W3148392242 cites W2076786023 @default.
• W3148392242 cites W2079042280 @default.
• W3148392242 cites W2079239113 @default.
• W3148392242 cites W2086415805 @default.
• W3148392242 cites W2092193862 @default.
• W3148392242 cites W2120693516 @default.
• W3148392242 cites W2121787561 @default.
• W3148392242 cites W2122935465 @default.
• W3148392242 cites W2128280157 @default.
• W3148392242 cites W2131258412 @default.
• W3148392242 cites W2131928928 @default.
• W3148392242 cites W2135760784 @default.
• W3148392242 cites W2139522433 @default.
• W3148392242 cites W2148942929 @default.
• W3148392242 cites W2149622622 @default.
• W3148392242 cites W2156554003 @default.
• W3148392242 cites W2161421542 @default.
• W3148392242 cites W2162674813 @default.
• W3148392242 cites W2164619944 @default.
• W3148392242 cites W2166972310 @default.
• W3148392242 cites W2334205457 @default.
• W3148392242 cites W59687201 @default.
• W3148392242 hasPublicationYear "2013" @default.
• W3148392242 type Work @default.
• W3148392242 sameAs 3148392242 @default.
• W3148392242 citedByCount "0" @default.
• W3148392242 crossrefType "journal-article" @default.
• W3148392242 hasAuthorship W3148392242A5005983180 @default.
• W3148392242 hasAuthorship W3148392242A5079319835 @default.
• W3148392242 hasConcept C104317684 @default.
• W3148392242 hasConcept C137620995 @default.
• W3148392242 hasConcept C148738053 @default.
• W3148392242 hasConcept C153911025 @default.
• W3148392242 hasConcept C190283241 @default.
• W3148392242 hasConcept C20875687 @default.
• W3148392242 hasConcept C26805974 @default.
• W3148392242 hasConcept C29537977 @default.
• W3148392242 hasConcept C54009773 @default.
• W3148392242 hasConcept C54355233 @default.
• W3148392242 hasConcept C553184892 @default.
• W3148392242 hasConcept C62112901 @default.
• W3148392242 hasConcept C62478195 @default.
• W3148392242 hasConcept C81885089 @default.
• W3148392242 hasConcept C86803240 @default.
• W3148392242 hasConcept C95444343 @default.
• W3148392242 hasConceptScore W3148392242C104317684 @default.
• W3148392242 hasConceptScore W3148392242C137620995 @default.
• W3148392242 hasConceptScore W3148392242C148738053 @default.
• W3148392242 hasConceptScore W3148392242C153911025 @default.
• W3148392242 hasConceptScore W3148392242C190283241 @default.
• W3148392242 hasConceptScore W3148392242C20875687 @default.
• W3148392242 hasConceptScore W3148392242C26805974 @default.
• W3148392242 hasConceptScore W3148392242C29537977 @default.
• W3148392242 hasConceptScore W3148392242C54009773 @default.
• W3148392242 hasConceptScore W3148392242C54355233 @default.
• W3148392242 hasConceptScore W3148392242C553184892 @default.
• W3148392242 hasConceptScore W3148392242C62112901 @default.
• W3148392242 hasConceptScore W3148392242C62478195 @default.
• W3148392242 hasConceptScore W3148392242C81885089 @default.
• W3148392242 hasConceptScore W3148392242C86803240 @default.
• W3148392242 hasConceptScore W3148392242C95444343 @default.
• W3148392242 hasLocation W31483922421 @default.
• W3148392242 hasOpenAccess W3148392242 @default.
• W3148392242 hasPrimaryLocation W31483922421 @default.
• W3148392242 hasRelatedWork W2496168162 @default.
• W3148392242 hasRelatedWork W2509302972 @default.
• W3148392242 hasRelatedWork W2510466893 @default.
• W3148392242 hasRelatedWork W2517943968 @default.
• W3148392242 hasRelatedWork W2603213172 @default.
• W3148392242 hasRelatedWork W2791672194 @default.
• W3148392242 hasRelatedWork W2800094342 @default.

• next