Matches in SemOpenAlex for { <https://semopenalex.org/work/W3148392330> ?p ?o ?g. }
- W3148392330 abstract "Abstract Background Breeding a mare until she is not fertile or even until her death is common in equine industry but the fertility decreases as the mare age increases. Embryo loss due to reduced embryo quality is partly accountable for this observation. Here, the effect of mare’s age on blastocysts’ gene expression was explored. Day 8 post-ovulation embryos were collected from multiparous young (YM, 6-year-old, N = 5) and older (OM, > 10-year-old, N = 6) non-nursing Saddlebred mares, inseminated with the semen of one stallion. Pure or inner cell mass (ICM) enriched trophoblast, obtained by embryo bisection, were RNA sequenced. Deconvolution algorithm was used to discriminate gene expression in the ICM from that in the trophoblast. Differential expression was analyzed with embryo sex and diameter as cofactors. Functional annotation and classification of differentially expressed genes and gene set enrichment analysis were also performed. Results Maternal aging did not affect embryo recovery rate, embryo diameter nor total RNA quantity. In both compartments, the expression of genes involved in mitochondria and protein metabolism were disturbed by maternal age, although more genes were affected in the ICM. Mitosis, signaling and adhesion pathways and embryo development were decreased in the ICM of embryos from old mares. In trophoblast, ion movement pathways were affected. Conclusions This is the first study showing that maternal age affects gene expression in the equine blastocyst, demonstrating significant effects as early as 10 years of age. These perturbations may affect further embryo development and contribute to decreased fertility due to aging." @default.
- W3148392330 created "2021-04-13" @default.
- W3148392330 creator A5006948865 @default.
- W3148392330 creator A5007577370 @default.
- W3148392330 creator A5007801214 @default.
- W3148392330 creator A5007964697 @default.
- W3148392330 creator A5013437443 @default.
- W3148392330 creator A5030171590 @default.
- W3148392330 creator A5039519752 @default.
- W3148392330 creator A5044634508 @default.
- W3148392330 creator A5057286572 @default.
- W3148392330 creator A5058604677 @default.
- W3148392330 creator A5070569841 @default.
- W3148392330 creator A5078689204 @default.
- W3148392330 creator A5089011370 @default.
- W3148392330 date "2021-04-09" @default.
- W3148392330 modified "2023-10-13" @default.
- W3148392330 title "Maternal age affects equine Day 8 embryo gene expression both in trophoblast and inner cell mass" @default.
- W3148392330 cites W1515473965 @default.
- W3148392330 cites W1570194759 @default.
- W3148392330 cites W1572456387 @default.
- W3148392330 cites W1576773659 @default.
- W3148392330 cites W1963209155 @default.
- W3148392330 cites W1970877777 @default.
- W3148392330 cites W1982877898 @default.
- W3148392330 cites W1984752631 @default.
- W3148392330 cites W1985379136 @default.
- W3148392330 cites W1989180582 @default.
- W3148392330 cites W1994777061 @default.
- W3148392330 cites W1996709275 @default.
- W3148392330 cites W2004140454 @default.
- W3148392330 cites W2008892561 @default.
- W3148392330 cites W2012787196 @default.
- W3148392330 cites W2014903163 @default.
- W3148392330 cites W2021341670 @default.
- W3148392330 cites W2022436116 @default.
- W3148392330 cites W2022492842 @default.
- W3148392330 cites W2023612761 @default.
- W3148392330 cites W2025116182 @default.
- W3148392330 cites W2025657442 @default.
- W3148392330 cites W2026579311 @default.
- W3148392330 cites W2030036440 @default.
- W3148392330 cites W2032450030 @default.
- W3148392330 cites W2034352799 @default.
- W3148392330 cites W2040244476 @default.
- W3148392330 cites W2042707253 @default.
- W3148392330 cites W2043821176 @default.
- W3148392330 cites W2045343796 @default.
- W3148392330 cites W2048001044 @default.
- W3148392330 cites W2058536248 @default.
- W3148392330 cites W2059650219 @default.
- W3148392330 cites W2060384891 @default.
- W3148392330 cites W2061229673 @default.
- W3148392330 cites W2062716171 @default.
- W3148392330 cites W2064387905 @default.
- W3148392330 cites W2065939516 @default.
- W3148392330 cites W2076170611 @default.
- W3148392330 cites W2077603983 @default.
- W3148392330 cites W2081870901 @default.
- W3148392330 cites W2085543001 @default.
- W3148392330 cites W2089139236 @default.
- W3148392330 cites W2094316551 @default.
- W3148392330 cites W2096536160 @default.
- W3148392330 cites W2104005232 @default.
- W3148392330 cites W2110194655 @default.
- W3148392330 cites W2121966499 @default.
- W3148392330 cites W2123106337 @default.
- W3148392330 cites W2123814691 @default.
- W3148392330 cites W2130071727 @default.
- W3148392330 cites W2130410032 @default.
- W3148392330 cites W2130451741 @default.
- W3148392330 cites W2133100663 @default.
- W3148392330 cites W2136657825 @default.
- W3148392330 cites W2138051350 @default.
- W3148392330 cites W2138207763 @default.
- W3148392330 cites W2152054207 @default.
- W3148392330 cites W2155753471 @default.
- W3148392330 cites W2159675211 @default.
- W3148392330 cites W2160573697 @default.
- W3148392330 cites W2169456326 @default.
- W3148392330 cites W2179438025 @default.
- W3148392330 cites W2181756887 @default.
- W3148392330 cites W2214074259 @default.
- W3148392330 cites W2287068230 @default.
- W3148392330 cites W2306318482 @default.
- W3148392330 cites W2417440641 @default.
- W3148392330 cites W2602663030 @default.
- W3148392330 cites W2735092275 @default.
- W3148392330 cites W2740170825 @default.
- W3148392330 cites W275222517 @default.
- W3148392330 cites W2754078695 @default.
- W3148392330 cites W2758215731 @default.
- W3148392330 cites W2806887119 @default.
- W3148392330 cites W2884564483 @default.
- W3148392330 cites W2888707991 @default.
- W3148392330 cites W2900449874 @default.
- W3148392330 cites W2903068484 @default.
- W3148392330 cites W2906135749 @default.
- W3148392330 cites W2942501466 @default.
- W3148392330 cites W2947990714 @default.