FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W3148416087> ?p ?o ?g. }

• W3148416087 endingPage "868" @default.
• W3148416087 startingPage "861" @default.
• W3148416087 abstract "MicroRNA-221 (miRNA-221) is upregulated in several malignant tumors and is associated with poor patient prognosis. Therefore, the present study aimed to investigate the role and underlying mechanism of miRNA-221 in doxorubicin (DOX) resistance in osteosarcoma cells. We constructed DOX-resistant Saos-2/DOX cells and treated them with DOX. Cell viability was determined by performing a 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. Cells were transfected with either miRNA-221 mimic or miRNA-221 inhibitor; quantitative (q)RT-PCR was performed to detect the expression of miRNA-221. Flow cytometry and terminal deoxynucleotidyl transferase-mediated deoxyuridine triphosphate-digoxigenin nick-end labeling (TUNEL) staining were used to detect cell apoptosis. The immunofluorescence method was also used to detect cell signal transduction and activator of transcription 3 (Stat3) protein expression distribution. In addition, Western blotting was used to detect changes in the expression of each protein. We found that miRNA-221 was upregulated in Saos-2/DOX cells. Moreover, the miRNA-221 mimic induced DOX resistance in Saos-2 cells, whereas the miRNA-221 inhibitor enhanced DOX sensitivity in Saos-2/DOX cells. The miRNA-221 mimic upregulated the expression of phosphorylated-Stat3, P-glycoprotein (P-gp), and B-cell lymphoma-2 (Bcl-2) proteins in Saos-2 cells and induced the entry of Stat3 into the nucleus, whereas the miRNA-221 inhibitor exerted the opposite effect. Pretreatment with the Stat3 chemical inhibitor, STAT3-IN-3, significantly inhibited the upregulation of P-gp and Bcl-2 protein expression induced by the miRNA-221 mimic in Saos-2 cells; it also caused the Saos-2 cells to overcome DOX resistance induced by the miRNA-221 mimic. Thus, miRNA-221 increased the expression of P-gp and Bcl-2 by activating the Stat3 pathway to promote DOX resistance in osteosarcoma cells, indicating a potential use of miRNA-221 in osteosarcoma treatment." @default.
• W3148416087 created "2021-04-13" @default.
• W3148416087 creator A5005193562 @default.
• W3148416087 creator A5069035448 @default.
• W3148416087 creator A5080902896 @default.
• W3148416087 date "2021-06-01" @default.
• W3148416087 modified "2023-10-11" @default.
• W3148416087 title "MicroRNA-221 Upregulates the Expression of P-gp and Bcl-2 by Activating the Stat3 Pathway to Promote Doxorubicin Resistance in Osteosarcoma Cells" @default.
• W3148416087 cites W1594210198 @default.
• W3148416087 cites W2012796350 @default.
• W3148416087 cites W2094935983 @default.
• W3148416087 cites W2138664009 @default.
• W3148416087 cites W2155582899 @default.
• W3148416087 cites W2264436887 @default.
• W3148416087 cites W2508325336 @default.
• W3148416087 cites W2521004297 @default.
• W3148416087 cites W2553067940 @default.
• W3148416087 cites W2560204073 @default.
• W3148416087 cites W2560965040 @default.
• W3148416087 cites W2592494468 @default.
• W3148416087 cites W2736106977 @default.
• W3148416087 cites W2778581470 @default.
• W3148416087 cites W2790958376 @default.
• W3148416087 cites W2792316773 @default.
• W3148416087 cites W2794160361 @default.
• W3148416087 cites W2794726454 @default.
• W3148416087 cites W2803684383 @default.
• W3148416087 cites W2805586774 @default.
• W3148416087 cites W2805942737 @default.
• W3148416087 cites W2884307168 @default.
• W3148416087 cites W2885740645 @default.
• W3148416087 cites W2888348740 @default.
• W3148416087 cites W2900248192 @default.
• W3148416087 cites W2901976265 @default.
• W3148416087 cites W2903454293 @default.
• W3148416087 cites W2903968876 @default.
• W3148416087 cites W2904033762 @default.
• W3148416087 cites W2906655844 @default.
• W3148416087 cites W2908222461 @default.
• W3148416087 cites W2912756443 @default.
• W3148416087 cites W2912847458 @default.
• W3148416087 cites W2941090871 @default.
• W3148416087 cites W2943663735 @default.
• W3148416087 cites W2975828557 @default.
• W3148416087 cites W2989873541 @default.
• W3148416087 cites W3146360245 @default.
• W3148416087 doi "https://doi.org/10.1248/bpb.b21-00163" @default.
• W3148416087 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/33828027" @default.
• W3148416087 hasPublicationYear "2021" @default.
• W3148416087 type Work @default.
• W3148416087 sameAs 3148416087 @default.
• W3148416087 citedByCount "12" @default.
• W3148416087 countsByYear W31484160872021 @default.
• W3148416087 countsByYear W31484160872022 @default.
• W3148416087 countsByYear W31484160872023 @default.
• W3148416087 crossrefType "journal-article" @default.
• W3148416087 hasAuthorship W3148416087A5005193562 @default.
• W3148416087 hasAuthorship W3148416087A5069035448 @default.
• W3148416087 hasAuthorship W3148416087A5080902896 @default.
• W3148416087 hasBestOaLocation W31484160871 @default.
• W3148416087 hasConcept C104317684 @default.
• W3148416087 hasConcept C127561419 @default.
• W3148416087 hasConcept C145059251 @default.
• W3148416087 hasConcept C153911025 @default.
• W3148416087 hasConcept C185592680 @default.
• W3148416087 hasConcept C190283241 @default.
• W3148416087 hasConcept C2778923194 @default.
• W3148416087 hasConcept C502942594 @default.
• W3148416087 hasConcept C53227056 @default.
• W3148416087 hasConcept C54009773 @default.
• W3148416087 hasConcept C54355233 @default.
• W3148416087 hasConcept C553184892 @default.
• W3148416087 hasConcept C55493867 @default.
• W3148416087 hasConcept C62478195 @default.
• W3148416087 hasConcept C81885089 @default.
• W3148416087 hasConcept C86803240 @default.
• W3148416087 hasConcept C95444343 @default.
• W3148416087 hasConceptScore W3148416087C104317684 @default.
• W3148416087 hasConceptScore W3148416087C127561419 @default.
• W3148416087 hasConceptScore W3148416087C145059251 @default.
• W3148416087 hasConceptScore W3148416087C153911025 @default.
• W3148416087 hasConceptScore W3148416087C185592680 @default.
• W3148416087 hasConceptScore W3148416087C190283241 @default.
• W3148416087 hasConceptScore W3148416087C2778923194 @default.
• W3148416087 hasConceptScore W3148416087C502942594 @default.
• W3148416087 hasConceptScore W3148416087C53227056 @default.
• W3148416087 hasConceptScore W3148416087C54009773 @default.
• W3148416087 hasConceptScore W3148416087C54355233 @default.
• W3148416087 hasConceptScore W3148416087C553184892 @default.
• W3148416087 hasConceptScore W3148416087C55493867 @default.
• W3148416087 hasConceptScore W3148416087C62478195 @default.
• W3148416087 hasConceptScore W3148416087C81885089 @default.
• W3148416087 hasConceptScore W3148416087C86803240 @default.
• W3148416087 hasConceptScore W3148416087C95444343 @default.
• W3148416087 hasIssue "6" @default.
• W3148416087 hasLocation W31484160871 @default.
• W3148416087 hasLocation W31484160872 @default.
• W3148416087 hasOpenAccess W3148416087 @default.

• next