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- W3148447653 abstract "The protoplasts of Volvariella volvacea V23 and V3552 were prepared by enzymatic method,and then were taken mutagenesis by UV and chemical 2 methods.A fusant displaying higher antifreeze capacity,as compared to that of its parents,was obtained by PEG-induced fusion between UV-inactivated protoplasts of V3552 EMS and heatinactivated protoplasts of V23 UV through low temperature(0℃) selection.The best fusion condition is gained at 400g /LPEG6000,pH 8.0,for 30min fusion time,at 32℃ fusion temperature.The fusion rate could be as high as 0.517%.And 200 fusants were obtained.Through screening with 0℃ low temperature,15 Volvariella volvacea cold tolerant bacterial strains were obtained.The cold tolerant ability of hypha at 0℃ was improved by 4.5 times.The results proved that the fruit displayed higher antifreeze capacity,as compared with bacterial strains;and the liquefaction phenomenon was obviously postponed.This indicated that strains screened by this method were of further development value." @default.
- W3148447653 created "2021-04-13" @default.
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- W3148447653 date "2013-01-01" @default.
- W3148447653 modified "2023-09-24" @default.
- W3148447653 title "Application of Inactivated Parental Strain Protoplasts Fusion Technology in Selection of Volvariella volvacea with Higher Antifreeze Capacity" @default.
- W3148447653 hasPublicationYear "2013" @default.
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