Matches in SemOpenAlex for { <https://semopenalex.org/work/W3148455688> ?p ?o ?g. }
- W3148455688 abstract "Abstract Transcription by RNA Polymerase II (Pol II) is initiated by the hierarchical assembly of the Pre-Initiation Complex onto promoter DNA. Decades of in vitro and yeast research have shown that the TATA-box binding protein (TBP) is essential to Pol II initiation by triggering the binding of other general transcription factors, and ensuring proper Pol II loading. Here, we report instead that acute depletion of TBP in mouse embryonic stem cells (mESCs) has no global effect on ongoing Pol II transcription. Surprisingly, Pol II transcriptional induction through the Heat Shock Response or cellular differentiation also occurs normally in the absence of TBP. In contrast, acute TBP depletion severely impairs initiation by RNA Polymerase III. Lastly, we show that a metazoan-specific paralog of TBP is expressed in mESCs and that it binds to promoter regions of active Pol II genes even in the absence of TBP. Taken together, our findings reveal an unexplored TBP-independent process in mESCs that points to a diversity in Pol II transcription initiation mechanisms." @default.
- W3148455688 created "2021-04-13" @default.
- W3148455688 creator A5013441790 @default.
- W3148455688 creator A5033949006 @default.
- W3148455688 creator A5039379693 @default.
- W3148455688 creator A5077805337 @default.
- W3148455688 creator A5079273275 @default.
- W3148455688 creator A5085368851 @default.
- W3148455688 date "2021-03-29" @default.
- W3148455688 modified "2023-09-27" @default.
- W3148455688 title "A TBP-independent mechanism for RNA Polymerase II transcription" @default.
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