Matches in SemOpenAlex for { <https://semopenalex.org/work/W3148457134> ?p ?o ?g. }
Showing items 1 to 69 of
69
with 100 items per page.
- W3148457134 abstract "A nitrous acid-induced, temperature sensitive mutant of cowpea mosaic virus(CPMV) known as 8-14, (Evans 1985, Virology 1985, 141, 275-282), wascharacterised. The phenotypic defect in 8 -14 was shown not to affect translationof the RNA or the first proteolytic cleavage of the B RNA-encoded polyprotein.The defect is probably at the level of genome replication. The technique of twodimensional RNA fingerprinting showed the mutant genome to be similar to theparental wild-type but did not resolve the genetic alteration(s) specific for themutation.The mechanism of CPMV translation was investigated by site-directedmutagenesis of a full-length cDNA clone of CPMV M RNA from which infectiousRNA could be generated by in vitro transcription. The results obtained confirmthe AUG at position 161 is used to direct the synthesis of the 105K proteinin vitro. The detection of a 58K protein in infected protoplasts suggests that itis also used in vivo. The synthesis of the 95K protein can be initiated fromeither of the AUGs at positions 512 and 524. Synthesis of this protein is notessential for CPMV replication in protoplasts.Several deletion mutations were created in the M RNA cDNA clone in order todetermine the regions of M RNA essential for replication of M RNA. Analysis ofone mutant indicated that sequences between 1446 and 1620 are probably notrequired for replicase recognition. However, the accumulation of this mutant inprotoplasts was reduced, presumably as a result of lack of encapsidation of theRNA as this mutant is thought not to synthesise functional coat protein. Datafrom several mutants showed that alterations of M RNA around nucleotides 161and 189 prevent transcript accumulation in protoplasts possibly owing to asevere reduction in replicability of the input RNA." @default.
- W3148457134 created "2021-04-13" @default.
- W3148457134 creator A5012942466 @default.
- W3148457134 date "1989-01-01" @default.
- W3148457134 modified "2023-09-27" @default.
- W3148457134 title "Isolation and characterisation of mutants of cowpea mosaic virus." @default.
- W3148457134 hasPublicationYear "1989" @default.
- W3148457134 type Work @default.
- W3148457134 sameAs 3148457134 @default.
- W3148457134 citedByCount "0" @default.
- W3148457134 crossrefType "dissertation" @default.
- W3148457134 hasAuthorship W3148457134A5012942466 @default.
- W3148457134 hasConcept C104317684 @default.
- W3148457134 hasConcept C138885662 @default.
- W3148457134 hasConcept C140704245 @default.
- W3148457134 hasConcept C143065580 @default.
- W3148457134 hasConcept C153911025 @default.
- W3148457134 hasConcept C159047783 @default.
- W3148457134 hasConcept C179926584 @default.
- W3148457134 hasConcept C2522874641 @default.
- W3148457134 hasConcept C2776541148 @default.
- W3148457134 hasConcept C3675279 @default.
- W3148457134 hasConcept C41258723 @default.
- W3148457134 hasConcept C41895202 @default.
- W3148457134 hasConcept C54355233 @default.
- W3148457134 hasConcept C67705224 @default.
- W3148457134 hasConcept C86803240 @default.
- W3148457134 hasConceptScore W3148457134C104317684 @default.
- W3148457134 hasConceptScore W3148457134C138885662 @default.
- W3148457134 hasConceptScore W3148457134C140704245 @default.
- W3148457134 hasConceptScore W3148457134C143065580 @default.
- W3148457134 hasConceptScore W3148457134C153911025 @default.
- W3148457134 hasConceptScore W3148457134C159047783 @default.
- W3148457134 hasConceptScore W3148457134C179926584 @default.
- W3148457134 hasConceptScore W3148457134C2522874641 @default.
- W3148457134 hasConceptScore W3148457134C2776541148 @default.
- W3148457134 hasConceptScore W3148457134C3675279 @default.
- W3148457134 hasConceptScore W3148457134C41258723 @default.
- W3148457134 hasConceptScore W3148457134C41895202 @default.
- W3148457134 hasConceptScore W3148457134C54355233 @default.
- W3148457134 hasConceptScore W3148457134C67705224 @default.
- W3148457134 hasConceptScore W3148457134C86803240 @default.
- W3148457134 hasLocation W31484571341 @default.
- W3148457134 hasOpenAccess W3148457134 @default.
- W3148457134 hasPrimaryLocation W31484571341 @default.
- W3148457134 hasRelatedWork W1570553982 @default.
- W3148457134 hasRelatedWork W1716872976 @default.
- W3148457134 hasRelatedWork W1767303762 @default.
- W3148457134 hasRelatedWork W1858596353 @default.
- W3148457134 hasRelatedWork W1966774557 @default.
- W3148457134 hasRelatedWork W1972610030 @default.
- W3148457134 hasRelatedWork W1981573676 @default.
- W3148457134 hasRelatedWork W1982105657 @default.
- W3148457134 hasRelatedWork W1997077753 @default.
- W3148457134 hasRelatedWork W2028654559 @default.
- W3148457134 hasRelatedWork W2039734963 @default.
- W3148457134 hasRelatedWork W2053978510 @default.
- W3148457134 hasRelatedWork W2076157501 @default.
- W3148457134 hasRelatedWork W2101319369 @default.
- W3148457134 hasRelatedWork W2105886687 @default.
- W3148457134 hasRelatedWork W2107242743 @default.
- W3148457134 hasRelatedWork W2129001132 @default.
- W3148457134 hasRelatedWork W2152852049 @default.
- W3148457134 hasRelatedWork W2172081967 @default.
- W3148457134 hasRelatedWork W251538164 @default.
- W3148457134 isParatext "false" @default.
- W3148457134 isRetracted "false" @default.
- W3148457134 magId "3148457134" @default.
- W3148457134 workType "dissertation" @default.