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- W3148481919 abstract "Objective To observe the effects of antisense transforming growth factor β receptor Ⅰ (T β RⅠ) expressing plasmid on rat liver fibrosis. Methods RT nested PCR and gene recombinant techniques were used to construct the rat antisense T β RⅠ recombinant plasmid which can be expressed in eucaryotic cells. The recombinant plasmid and blanx vector (pcDNA3) were encapsulated by glycosyl poly L lysine and then transducted into rats of pig serum induced liver fibrosis model respectively. Expression of exogenous transfected plasmid was assessed by Northern blot, RT PCR ,Western blot and immunohistochemistry. Serum TGF β 1 was determined by ELISA. The content of hepatic hydroxyproline was tested. Immunohistochemistry was used to test type I and III collagen. VG staining was used for pathological study. Results The exogenous antisense T β RⅠcould be expressed in vivo, and could block the mRNA and protein expression of T β RⅠin the fibrotic liver induced by pig serum. Its expression also reduced the level of TGF β 1 ( P 0.05) and decreased the the contents of hepatic hydroxyproline and the deposition of collagens type I and type III ( P 0.01). And its expression also improved the pathologic classification of liver fibrosis models ( P 0.01). Conclusions The results demonstrate that the antisense T β RⅠ recombinant plasmid has certain extern reverse effect on liver fibrosis and make it as a possible candidate for use in future gene therapy." @default.
- W3148481919 created "2021-04-13" @default.
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- W3148481919 date "2002-01-01" @default.
- W3148481919 modified "2023-09-23" @default.
- W3148481919 title "Effects of antisense transforming growth factor β receptor-I expressing plasmid on pig serum-induced rat liver fibrosis" @default.
- W3148481919 hasPublicationYear "2002" @default.
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