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- W3148484534 abstract "To observe the effect of electroacupuncture (EA) on the expression of soluble guanylate cyclase (sGC), cyclic guanosine phosphate (cGMP) and protein kinase G (PKG) of cerebral vascular smooth muscle in cerebral infarction (CI) rats, so as to study its dynamic regulation mechanism.Male Wistar rats were randomly divided into normal control (n=10), sham operation (n=40), model (n=40), and EA (n=40) groups, and the latter three groups were further di-vided into 3, 6, 12 and 24 h subgroups (n=10 in each subgroup). The CI model was established by occlusion of the middle cerebral artery (MCAO). EA(15 Hz, 2 mA)was applied to Shuigou (GV26) for 20 min. The cGMP, sGC and PKG activity and expression levels in the vascular smooth muscle of cerebral artery were detected using ELISA and Western blot, respectively.After modeling, the immunoactivity and activities of sGC at 3 h, PKG at 3 and 6 h and cGMP from 3 h to 24 h were ob-viously decreased in the model group relevant to the normal control and sham-operation groups (P<0.05, P<0.01). After the intervention, the expression levels and activities of sGC at 3 h, PKG at 3 and 6 h and cGMP at 3 and 6 h were apparently up-regulated in the EA group relevant to the model group (P<0.05).EA intervention can significantly inhibit the down-regulation of sGC, PKG and cGMP expression of cerebral artery smooth muscle in MCAO model rats, which plays an important role in inhibiting cerebral artery smooth muscle spasm after ischemia, maintaining normal vascular function and state, and thus increasing blood perfusion around cerebral infarction area. However, acupuncture effect has a certain time-effectiveness.目的：观察电针“水沟”对脑梗死大鼠脑动脉血管平滑肌可溶性鸟苷酸环化酶(sGC)、蛋白激酶G(PKG)及环磷酸鸟苷(cGMP)变化的动态调节规律。方法：Wistar大鼠随机分为空白组(10只)、假手术组(40只)、模型组(40只)、电针组(40只)，后3组在造模后又分为3、6、12、24 h 4个时相组，每组10只。以线栓法复制脑梗死大鼠模型。电针组在造模后即刻针刺“水沟”，电针刺激20 min。各组动物均按时相处死，应用蛋白免疫印迹法、酶联免疫吸附法分别检测脑动脉血管平滑肌sGC、PKG及cGMP的变化。结果：大鼠脑梗死后24 h内，与空白组和假手术组比较，模型组3 h时相的sGC表达水平显著下调(P<0.01)，且sGC的整体表达量随梗死时间的延长有逐渐升高的趋势，3、6 h时相的PKG表达量显著下调(P<0.05，P<0.01)，各时相的cGMP含量均显著下降(P<0.01)。与同时相模型组比较，电针组3 h时相的sGC表达水平明显上调(P<0.05)，3、6 h时相的PKG表达水平上调(P<0.05)，3、6 h的cGMP含量增加(P<0.05)。结论：针刺干预能显著抑制脑梗死模型大鼠脑动脉血管平滑肌sGC、PKG、cGMP表达下调，这对于抑制缺血后脑动脉血管平滑肌痉挛，保持血管功能和状态的正常，从而增加脑梗死区周围血流灌注具有重要作用，但针刺效应具有一定时效性。." @default.
- W3148484534 created "2021-04-13" @default.
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- W3148484534 date "2020-10-25" @default.
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- W3148484534 title "[Effects of electroacupuncture Shuigou on expression of soluble guanylate cyclase(sGC) and protein kinase G (PKG)in vascular smooth muscle of cerebral artery in rats with cerebral infarction]." @default.
- W3148484534 doi "https://doi.org/10.13702/j.1000-0607.200076" @default.
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