Matches in SemOpenAlex for { <https://semopenalex.org/work/W3148519265> ?p ?o ?g. }
Showing items 1 to 61 of
61
with 100 items per page.
- W3148519265 abstract "Objective To produce anti‐19‐Nortestosterone (NT) monoclonal antibodies and identify their immunological characteristics. Methods Hybridomas were prepared by fusing NS0 mouse myeloma cells with splenocytes isolated from immunized BALB/c mice. Noncompetitive and competitive indirect ELISA were employed to screen positive cell clones. A caprylic acid ammonium sulphate (CAAP) method was used to purify NT mAb, and the Batty saturation method was used to determine the affinity constant (Kaff). Results Five hybridoma cell lines, named NT‐1, NT‐2, NT‐3, NT‐4, and NT‐5, were identified and their corresponding mAbs were of the IgG 1 isotype with a k light chain. The Kaffs of all mAbs were between 2.6 and 4.7×10 9 L/mol. The titers and IC 50 values of purified ascite fluids were in the range of (0.64–2.56)×10 5 and (0.55–1.0) ng/mL, respectively. Of all the cross‐reacting steroids, α ‐NT was the most reactive with the mAbs at 62% with NT‐1 mAb and 64% with NT‐2 mAb. Negligible cross‐reactivity (0.01%) with other steroids was observed. Conclusion The establishment of these hybridomas allows the potential development of a rapid test kit, and may provide an alternative method for the detection of NT residues in food producing animals." @default.
- W3148519265 created "2021-04-13" @default.
- W3148519265 creator A5029871478 @default.
- W3148519265 date "2011-01-01" @default.
- W3148519265 modified "2023-09-25" @default.
- W3148519265 title "Production and Characterisation of Monoclonal Antibodies against 19-Nortestosterone" @default.
- W3148519265 hasPublicationYear "2011" @default.
- W3148519265 type Work @default.
- W3148519265 sameAs 3148519265 @default.
- W3148519265 citedByCount "0" @default.
- W3148519265 crossrefType "journal-article" @default.
- W3148519265 hasAuthorship W3148519265A5029871478 @default.
- W3148519265 hasConcept C153911025 @default.
- W3148519265 hasConcept C159654299 @default.
- W3148519265 hasConcept C185592680 @default.
- W3148519265 hasConcept C189976730 @default.
- W3148519265 hasConcept C202751555 @default.
- W3148519265 hasConcept C203014093 @default.
- W3148519265 hasConcept C2777991845 @default.
- W3148519265 hasConcept C32611913 @default.
- W3148519265 hasConcept C542903549 @default.
- W3148519265 hasConcept C55493867 @default.
- W3148519265 hasConcept C86803240 @default.
- W3148519265 hasConceptScore W3148519265C153911025 @default.
- W3148519265 hasConceptScore W3148519265C159654299 @default.
- W3148519265 hasConceptScore W3148519265C185592680 @default.
- W3148519265 hasConceptScore W3148519265C189976730 @default.
- W3148519265 hasConceptScore W3148519265C202751555 @default.
- W3148519265 hasConceptScore W3148519265C203014093 @default.
- W3148519265 hasConceptScore W3148519265C2777991845 @default.
- W3148519265 hasConceptScore W3148519265C32611913 @default.
- W3148519265 hasConceptScore W3148519265C542903549 @default.
- W3148519265 hasConceptScore W3148519265C55493867 @default.
- W3148519265 hasConceptScore W3148519265C86803240 @default.
- W3148519265 hasLocation W31485192651 @default.
- W3148519265 hasOpenAccess W3148519265 @default.
- W3148519265 hasPrimaryLocation W31485192651 @default.
- W3148519265 hasRelatedWork W1519852704 @default.
- W3148519265 hasRelatedWork W1981615336 @default.
- W3148519265 hasRelatedWork W2351021548 @default.
- W3148519265 hasRelatedWork W2351278371 @default.
- W3148519265 hasRelatedWork W2355295170 @default.
- W3148519265 hasRelatedWork W2356338171 @default.
- W3148519265 hasRelatedWork W2356748887 @default.
- W3148519265 hasRelatedWork W2365579196 @default.
- W3148519265 hasRelatedWork W2368180233 @default.
- W3148519265 hasRelatedWork W2370751708 @default.
- W3148519265 hasRelatedWork W2372099341 @default.
- W3148519265 hasRelatedWork W2372595495 @default.
- W3148519265 hasRelatedWork W2381400438 @default.
- W3148519265 hasRelatedWork W2383837903 @default.
- W3148519265 hasRelatedWork W2388873006 @default.
- W3148519265 hasRelatedWork W2392547907 @default.
- W3148519265 hasRelatedWork W2397480457 @default.
- W3148519265 hasRelatedWork W2401647108 @default.
- W3148519265 hasRelatedWork W2409899842 @default.
- W3148519265 hasRelatedWork W2978947898 @default.
- W3148519265 isParatext "false" @default.
- W3148519265 isRetracted "false" @default.
- W3148519265 magId "3148519265" @default.
- W3148519265 workType "article" @default.