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- W3148621533 abstract "Objective To establish real-time PCR TaqMan assay for the direct detection of Campylobacter jejuni from stool samples.Methods Two pairs of specific primers and probes were designed by hipO and mapA genes respectively.On the basis of evaluation of sensitivity,specificity and reproducibility of two systems,real-time PCR was performed to identify DNA extracted from 45 stool samples of clinical diarrhea patients and conventional culture was conducted too.Results C.jujeni strain could be detected by the 2 pairs of primers and probes with the detection limit of 10-20 cfu/ml.There was no cross-reactions with other enteric pathogens.Of 45 stool sample,3 were positive by real-time PCR,and 2 of which were positive by conventional pure culture.Conclusion Realtime PCR TaqMan assay in this study was highly sensitive and specific,which could rapidly detect C.jejuni from stool samples with high positive rate and in a shorter time." @default.
- W3148621533 created "2021-04-13" @default.
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- W3148621533 date "2009-01-01" @default.
- W3148621533 modified "2023-09-23" @default.
- W3148621533 title "Application of real-time PCR TaqMan assay for rapid detection of Campylobacter jejuni from stool samples" @default.
- W3148621533 hasPublicationYear "2009" @default.
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