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- W3148687334 abstract "The application of a new fluorogenic probe-based PCR assay (PCR duplex scorpion primer assay) to the detec- tion of Hepatitis B virus (HBV) DNA in human sera was described. Duplex scorpion primer is a modified variant of duplex Amplifluor, and the incorporation of a PCR stopper between probe and primer sequences improve the detec- tion specificity and sensitivity. Combined with PCR amplification, this probe can give unambiguous positive results for the reactions initiated with more than 20 HBV molecu les. In addition, the partic ular unimolecular probing mechanism of this probe makes the use of short target-specific probe seque nce possible, which will render this probe applicable in some specific systems." @default.
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- W3148687334 date "2004-01-01" @default.
- W3148687334 modified "2023-09-24" @default.
- W3148687334 title "Detection of Hepatitis B Virus DNA by Duplex Scorpion Primer-based PCR Assay †" @default.
- W3148687334 hasPublicationYear "2004" @default.
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