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- W3148747079 abstract "AIM To observe the histocompatibility and degradation of porcine decellular aortic valve (PDAV) without glutaradehyde treatment, so as to study the feasibility to construct a tissue engineered heart valve (TEHV). METHODS PDAVs seeded with canine aortic wall interstitial cells and endothelial cells (ECs) were implanted into canine abdominal aorta to observe their morphologic and immunohistochemistry changes. RESULTS ① Subcutaneous implantation of PDAV showed mild inflammatory response, and the subcutaneous degradation duration was 10 weeks. ② Canine aortic valve interstitial cells and ECs were seeded on PDAV respectively in vitro. Seeding cells appeared on the surface of PDAV after 7 days. ③ In vivo , the PDAV leaflets implanted in abdominal aorta were substituted gradually by the interstial connective tissue synthesized and secreted by the new cells, and were reconstructed completely at the end of the 10th weeks. Collagen type Ⅰ, Ⅲ, elastin and glycosaminoglycans were detected in the newly formed leaflets. The interstital cells with or without positive expression of α SMA in the leaflets and ECs with positive expression Ⅷ factors lining the suface of the leaflet were seen. CONCLUSION Primarily living cell leaflets in the constructed tissue engineered valves with PDAV in vitro which implanted in canine abdominal aorta for 10 weeks have been formed. The histocompatibility was superior to that of artifitial synthesized materials." @default.
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- W3148747079 date "2002-01-01" @default.
- W3148747079 modified "2023-09-23" @default.
- W3148747079 title "Experimental study on construction of tissue-engineered heart valve using porcine decellular aortic valve" @default.
- W3148747079 hasPublicationYear "2002" @default.
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