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- W3148805095 abstract "Objective To prepare the antiserum against antifreeze protein,MpAFP698,from the beetle Microdera punctipennis(M.punctipennis) and determine immunological homology of different antifreeze proteins from different insects in Tenebrionidae.Methods Two New Zealand white rabbits were immunized by DNA prime-protein boost strategy.Firstly, recombinant plasmid pcDNA3-Mpafp698 was used as DNA vaccine to inoculate two rabbits respectively for two times.Then twice boost immunization was performed using recombinant protein His-MpAFP698.ELISA and Western blotting were respectively conducted to determine the titer of MpAFP698 antibody and to detect the specificity of the antibody as well as the immunological cross-reaction of different insect antifreeze proteins with MpAFP698 antibody.Results The titer of the rabbit antiserum reached 1:400 000.Western blotting showed the specific binding of the antiserum to His-MpAFP698 protein and GST-MpAFP698 protein respectively.The rabbit antiserum also presented specific binding to antifreeze protein MpAFP149 and MpAFPS77 from M.punctipennis,as well as Apafp914 and OcAFP4 from different species.Conclusion Antifreeze proteins from different beetles in desert region have the same epitope and thus have immunological cross-reaction." @default.
- W3148805095 created "2021-04-13" @default.
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- W3148805095 date "2013-01-01" @default.
- W3148805095 modified "2023-09-24" @default.
- W3148805095 title "Immuno-cross-reactivity of different antifreeze proteins from Tenebrionidae insects" @default.
- W3148805095 hasPublicationYear "2013" @default.
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