Matches in SemOpenAlex for { <https://semopenalex.org/work/W3148808174> ?p ?o ?g. }
Showing items 1 to 92 of
92
with 100 items per page.
- W3148808174 endingPage "104851" @default.
- W3148808174 startingPage "104851" @default.
- W3148808174 abstract "Mycoplasma synoviae (MS) is an important pathogen which causes huge economic losses to the poultry industry worldwide, and research on MS can provide the foundation for diagnosis, prevention, and treatment of MS infection. In this study, primers designed based on the sequences of pyruvate dehydrogenase complex (PDC) E1 alpha and beta subunit genes (pdhA and pdhB, respectively) of MS 53 strain(AE017245.1) in GenBank were used to amplify the pdhA and pdhB genes of MS WVU1853 strain through PCR. Subsequently, the prokaryotic expression vectors pET-28a(+)-pdhA and pET-28a(+)-pdhB were constructed and expressed in Escherichia coli BL21(DE3) cells. The recombinant proteins rMSPDHA and rMSPDHB were purified, and anti-rMSPDHA and anti-rMSPDHB sera were prepared by immunizing rabbits, respectively. Subcellular localization of PDHA and PDHB in MS cells, binding activity of rMSPDHA and rMSPDHB to chicken plasminogen (Plg) and human fibronectin (Fn), complement-dependent mycoplasmacidal assays, and adherence and adherence inhibition assays were accomplished. The results showed that PDHA and PDHB were distributed both on the surface membrane and within soluble cytosolic fractions of MS cells. The rMSPDHA and rMSPDHB presented binding activity with chicken Plg and human Fn. The rabbit anti-rMSPDHA and anti-rMSPDHB sera had distinct mycoplasmacidal efficacy in the presence of guinea pig complement, and the adherence of MS to DF-1 cells pretreated with Plg was effectively inhibited by treatment with anti-rMSPDHA or anti-rMSPDHB sera. These findings indicated that surface-associated MSPDHA and MSPDHB were adhesion-related factors of MS and that the binding between MSPDHA/MSPDHB and Plg/Fn contributed to MS adhesion to DF-1 cells." @default.
- W3148808174 created "2021-04-13" @default.
- W3148808174 creator A5007024868 @default.
- W3148808174 creator A5012247397 @default.
- W3148808174 creator A5065570155 @default.
- W3148808174 creator A5070992149 @default.
- W3148808174 creator A5086300669 @default.
- W3148808174 date "2021-06-01" @default.
- W3148808174 modified "2023-10-16" @default.
- W3148808174 title "Characterization of pyruvate dehydrogenase complex E1 alpha and beta subunits of Mycoplasma synoviae" @default.
- W3148808174 cites W1517812011 @default.
- W3148808174 cites W1519352442 @default.
- W3148808174 cites W1776749002 @default.
- W3148808174 cites W1966553114 @default.
- W3148808174 cites W1977197046 @default.
- W3148808174 cites W1979105917 @default.
- W3148808174 cites W1980675669 @default.
- W3148808174 cites W1999458074 @default.
- W3148808174 cites W2019545199 @default.
- W3148808174 cites W2026161967 @default.
- W3148808174 cites W2037567350 @default.
- W3148808174 cites W2042478578 @default.
- W3148808174 cites W2061202598 @default.
- W3148808174 cites W2062868513 @default.
- W3148808174 cites W2068865134 @default.
- W3148808174 cites W2073571619 @default.
- W3148808174 cites W2076862889 @default.
- W3148808174 cites W2079338761 @default.
- W3148808174 cites W2081148357 @default.
- W3148808174 cites W2088417711 @default.
- W3148808174 cites W2089995257 @default.
- W3148808174 cites W2104901693 @default.
- W3148808174 cites W2135135191 @default.
- W3148808174 cites W2162306731 @default.
- W3148808174 cites W2164741370 @default.
- W3148808174 cites W2522533895 @default.
- W3148808174 cites W2556204257 @default.
- W3148808174 cites W2888671782 @default.
- W3148808174 cites W2035010296 @default.
- W3148808174 doi "https://doi.org/10.1016/j.micpath.2021.104851" @default.
- W3148808174 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/33794298" @default.
- W3148808174 hasPublicationYear "2021" @default.
- W3148808174 type Work @default.
- W3148808174 sameAs 3148808174 @default.
- W3148808174 citedByCount "1" @default.
- W3148808174 countsByYear W31488081742022 @default.
- W3148808174 crossrefType "journal-article" @default.
- W3148808174 hasAuthorship W3148808174A5007024868 @default.
- W3148808174 hasAuthorship W3148808174A5012247397 @default.
- W3148808174 hasAuthorship W3148808174A5065570155 @default.
- W3148808174 hasAuthorship W3148808174A5070992149 @default.
- W3148808174 hasAuthorship W3148808174A5086300669 @default.
- W3148808174 hasBestOaLocation W31488081742 @default.
- W3148808174 hasConcept C104292427 @default.
- W3148808174 hasConcept C104317684 @default.
- W3148808174 hasConcept C153911025 @default.
- W3148808174 hasConcept C40767141 @default.
- W3148808174 hasConcept C547475151 @default.
- W3148808174 hasConcept C55493867 @default.
- W3148808174 hasConcept C86803240 @default.
- W3148808174 hasConcept C89423630 @default.
- W3148808174 hasConceptScore W3148808174C104292427 @default.
- W3148808174 hasConceptScore W3148808174C104317684 @default.
- W3148808174 hasConceptScore W3148808174C153911025 @default.
- W3148808174 hasConceptScore W3148808174C40767141 @default.
- W3148808174 hasConceptScore W3148808174C547475151 @default.
- W3148808174 hasConceptScore W3148808174C55493867 @default.
- W3148808174 hasConceptScore W3148808174C86803240 @default.
- W3148808174 hasConceptScore W3148808174C89423630 @default.
- W3148808174 hasFunder F4320321001 @default.
- W3148808174 hasLocation W31488081741 @default.
- W3148808174 hasLocation W31488081742 @default.
- W3148808174 hasLocation W31488081743 @default.
- W3148808174 hasOpenAccess W3148808174 @default.
- W3148808174 hasPrimaryLocation W31488081741 @default.
- W3148808174 hasRelatedWork W1820517293 @default.
- W3148808174 hasRelatedWork W1996629794 @default.
- W3148808174 hasRelatedWork W1999490032 @default.
- W3148808174 hasRelatedWork W2016628627 @default.
- W3148808174 hasRelatedWork W2019922031 @default.
- W3148808174 hasRelatedWork W2073954518 @default.
- W3148808174 hasRelatedWork W2350869260 @default.
- W3148808174 hasRelatedWork W2418132576 @default.
- W3148808174 hasRelatedWork W3046976449 @default.
- W3148808174 hasRelatedWork W39854491 @default.
- W3148808174 hasVolume "155" @default.
- W3148808174 isParatext "false" @default.
- W3148808174 isRetracted "false" @default.
- W3148808174 magId "3148808174" @default.
- W3148808174 workType "article" @default.