FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W3148897605> ?p ?o ?g. }

• W3148897605 endingPage "3489" @default.
• W3148897605 startingPage "3489" @default.
• W3148897605 abstract "Glioblastoma (GBM) is the most common and aggressive primary malignant brain tumor in adults. Natural Killer (NK) cells are potent cytotoxic effector cells against tumor cells inducing GBM cells; therefore, NK cell based- immunotherapy might be a promising target in GBM. T cell immunoglobulin mucin family member 3 (TIM3), a receptor expressed on NK cells, has been suggested as a marker of dysfunctional NK cells. We established TIM3 knockout in NK cells, using the clustered regularly interspaced short palindromic repeats (CRISPR)-CRISPR-associated protein 9 (Cas9). Electroporating of TIM3 exon 2- or exon 5-targeting guide RNA- Cas9 protein complexes (RNPs) inhibited TIM3 expression on NK cells with varying efficacy. T7 endonuclease I mutation detection assays showed that both RNPs disrupted the intended genome sites. The expression of other checkpoint receptors, i.e., programmed cell death 1 (PD1), Lymphocyte-activation gene 3 (LAG3), T cell immunoreceptor with Ig and ITIM domains (TIGIT), and TACTILE (CD96) were unchanged on the TIM3 knockout NK cells. Real time cell growth assays revealed that TIM3 knockout enhanced NK cell–mediated growth inhibition of GBM cells. These results demonstrated that TIM3 knockout enhanced human NK cell mediated cytotoxicity on GBM cells. Future, CRISPR-Cas9 mediated TIM3 knockout in NK cells may prove to be a promising immunotherapeutic alternative in patient with GBM." @default.
• W3148897605 created "2021-04-13" @default.
• W3148897605 creator A5002163440 @default.
• W3148897605 creator A5018526643 @default.
• W3148897605 creator A5038389153 @default.
• W3148897605 creator A5045586849 @default.
• W3148897605 creator A5060268134 @default.
• W3148897605 creator A5060916666 @default.
• W3148897605 creator A5063373940 @default.
• W3148897605 creator A5078888657 @default.
• W3148897605 creator A5080351529 @default.
• W3148897605 creator A5087179801 @default.
• W3148897605 date "2021-03-28" @default.
• W3148897605 modified "2023-10-09" @default.
• W3148897605 title "CRISPR-Cas9–Mediated TIM3 Knockout in Human Natural Killer Cells Enhances Growth Inhibitory Effects on Human Glioma Cells" @default.
• W3148897605 cites W1481317976 @default.
• W3148897605 cites W1541265927 @default.
• W3148897605 cites W1828402880 @default.
• W3148897605 cites W1968791512 @default.
• W3148897605 cites W1996032846 @default.
• W3148897605 cites W1996890691 @default.
• W3148897605 cites W2005091069 @default.
• W3148897605 cites W2018325561 @default.
• W3148897605 cites W2020541351 @default.
• W3148897605 cites W2022580424 @default.
• W3148897605 cites W2023082289 @default.
• W3148897605 cites W2042364269 @default.
• W3148897605 cites W2046409157 @default.
• W3148897605 cites W2062961963 @default.
• W3148897605 cites W2066671159 @default.
• W3148897605 cites W2068047393 @default.
• W3148897605 cites W2073300038 @default.
• W3148897605 cites W2096287682 @default.
• W3148897605 cites W2096682838 @default.
• W3148897605 cites W2107777135 @default.
• W3148897605 cites W2112400160 @default.
• W3148897605 cites W2113863168 @default.
• W3148897605 cites W2114530568 @default.
• W3148897605 cites W2117973220 @default.
• W3148897605 cites W2122730216 @default.
• W3148897605 cites W2146007051 @default.
• W3148897605 cites W2167717479 @default.
• W3148897605 cites W2329107713 @default.
• W3148897605 cites W2332535881 @default.
• W3148897605 cites W2366536035 @default.
• W3148897605 cites W2403400382 @default.
• W3148897605 cites W2425588479 @default.
• W3148897605 cites W2479943477 @default.
• W3148897605 cites W2520704033 @default.
• W3148897605 cites W2533926722 @default.
• W3148897605 cites W2554863679 @default.
• W3148897605 cites W2595863515 @default.
• W3148897605 cites W2751720802 @default.
• W3148897605 cites W2787835497 @default.
• W3148897605 cites W2793648416 @default.
• W3148897605 cites W2799415594 @default.
• W3148897605 cites W2805359783 @default.
• W3148897605 cites W2911364339 @default.
• W3148897605 cites W2911752971 @default.
• W3148897605 cites W2921252083 @default.
• W3148897605 cites W2953102624 @default.
• W3148897605 cites W2985205513 @default.
• W3148897605 cites W3017329354 @default.
• W3148897605 cites W3026551532 @default.
• W3148897605 cites W3030339055 @default.
• W3148897605 cites W3032465961 @default.
• W3148897605 cites W3034974614 @default.
• W3148897605 cites W3042806452 @default.
• W3148897605 cites W3047587323 @default.
• W3148897605 cites W3115869600 @default.
• W3148897605 doi "https://doi.org/10.3390/ijms22073489" @default.
• W3148897605 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/8036491" @default.
• W3148897605 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/33800561" @default.
• W3148897605 hasPublicationYear "2021" @default.
• W3148897605 type Work @default.
• W3148897605 sameAs 3148897605 @default.
• W3148897605 citedByCount "28" @default.
• W3148897605 countsByYear W31488976052021 @default.
• W3148897605 countsByYear W31488976052022 @default.
• W3148897605 countsByYear W31488976052023 @default.
• W3148897605 crossrefType "journal-article" @default.
• W3148897605 hasAuthorship W3148897605A5002163440 @default.
• W3148897605 hasAuthorship W3148897605A5018526643 @default.
• W3148897605 hasAuthorship W3148897605A5038389153 @default.
• W3148897605 hasAuthorship W3148897605A5045586849 @default.
• W3148897605 hasAuthorship W3148897605A5060268134 @default.
• W3148897605 hasAuthorship W3148897605A5060916666 @default.
• W3148897605 hasAuthorship W3148897605A5063373940 @default.
• W3148897605 hasAuthorship W3148897605A5078888657 @default.
• W3148897605 hasAuthorship W3148897605A5080351529 @default.
• W3148897605 hasAuthorship W3148897605A5087179801 @default.
• W3148897605 hasBestOaLocation W31488976051 @default.
• W3148897605 hasConcept C104317684 @default.
• W3148897605 hasConcept C129374314 @default.
• W3148897605 hasConcept C153911025 @default.
• W3148897605 hasConcept C154317977 @default.
• W3148897605 hasConcept C202751555 @default.
• W3148897605 hasConcept C203014093 @default.

• next