Matches in SemOpenAlex for { <https://semopenalex.org/work/W3149087746> ?p ?o ?g. }
Showing items 1 to 63 of
63
with 100 items per page.
- W3149087746 abstract "textabstractSeveral methods are proposed for the elemental analysis of biologicalmaterial with the use of electron energy-loss spectroscopy (EELS) and electronspectroscopic imaging (ESI) in a Zeiss EM902.For spectra, the Simplex-method was tested and compared to Egerton's 2-area method, the Steepest descent method and the Loglog method. The Simplexmethod and the Loglog method performed best. The Simplex method was then usedfor the analysis of biological specimens containing calcium and iron. The results ofthe analysis of a test-spectrum, an iron-spectrum and a calcium-spectrum takenwith various widths of the r -region were compared. It was observed that calcium isa hard element for EELS-analysis because of the carbon K-edge just in front of itsionization-edge. Ca- and Fe-Bio-standards, which were co-embedded with thespecimens, were used to obtain elemental concentrations in the specimen.In combination with the Kontron IBAS 2000, morphometrical image analysiswas performed on cells and their reaction products. The influence of severalacquisition factors was analyzed (objective-lens diaphragm diameter, image mode,number of integrations during image acquisition). Segmentation is applied using thecross-overs of the frrst derivative of the greyvalue distribution histogram of theimage. Co-localization of several chemical elements could be demonstrated bycomparing the net-intensity distribution images of the respective elements.In images, several methods were tested for the separation of the elementinformationfrom the continuum. The power-law method gives the best results. Theinfluence of the width of the integration-region on the obtained relativeconcentration was tested.Since the power-law method did not overcome all problems encounteredwith image-analysis (Calcium), Multivariate statistical analysis (MSA) wasexanrined as an alternative. The latter method performed very well and was able toanalyze calcium images. MSA also reduces the influence of other sources ofinformation in the images such as shading and radiation damage" @default.
- W3149087746 created "2021-04-13" @default.
- W3149087746 creator A5073933114 @default.
- W3149087746 date "1993-06-30" @default.
- W3149087746 modified "2023-09-26" @default.
- W3149087746 title "Analysis of electron energy-loss spectra and images" @default.
- W3149087746 hasPublicationYear "1993" @default.
- W3149087746 type Work @default.
- W3149087746 sameAs 3149087746 @default.
- W3149087746 citedByCount "0" @default.
- W3149087746 crossrefType "journal-article" @default.
- W3149087746 hasAuthorship W3149087746A5073933114 @default.
- W3149087746 hasConcept C113196181 @default.
- W3149087746 hasConcept C121332964 @default.
- W3149087746 hasConcept C1276947 @default.
- W3149087746 hasConcept C145148216 @default.
- W3149087746 hasConcept C178790620 @default.
- W3149087746 hasConcept C185592680 @default.
- W3149087746 hasConcept C192562407 @default.
- W3149087746 hasConcept C198291218 @default.
- W3149087746 hasConcept C33923547 @default.
- W3149087746 hasConcept C43617362 @default.
- W3149087746 hasConcept C4839761 @default.
- W3149087746 hasConcept C64885871 @default.
- W3149087746 hasConceptScore W3149087746C113196181 @default.
- W3149087746 hasConceptScore W3149087746C121332964 @default.
- W3149087746 hasConceptScore W3149087746C1276947 @default.
- W3149087746 hasConceptScore W3149087746C145148216 @default.
- W3149087746 hasConceptScore W3149087746C178790620 @default.
- W3149087746 hasConceptScore W3149087746C185592680 @default.
- W3149087746 hasConceptScore W3149087746C192562407 @default.
- W3149087746 hasConceptScore W3149087746C198291218 @default.
- W3149087746 hasConceptScore W3149087746C33923547 @default.
- W3149087746 hasConceptScore W3149087746C43617362 @default.
- W3149087746 hasConceptScore W3149087746C4839761 @default.
- W3149087746 hasConceptScore W3149087746C64885871 @default.
- W3149087746 hasLocation W31490877461 @default.
- W3149087746 hasOpenAccess W3149087746 @default.
- W3149087746 hasPrimaryLocation W31490877461 @default.
- W3149087746 hasRelatedWork W1484717504 @default.
- W3149087746 hasRelatedWork W1517900726 @default.
- W3149087746 hasRelatedWork W1567090874 @default.
- W3149087746 hasRelatedWork W1967456192 @default.
- W3149087746 hasRelatedWork W2040356538 @default.
- W3149087746 hasRelatedWork W2065203671 @default.
- W3149087746 hasRelatedWork W2075951012 @default.
- W3149087746 hasRelatedWork W2076806971 @default.
- W3149087746 hasRelatedWork W2113786404 @default.
- W3149087746 hasRelatedWork W2120056293 @default.
- W3149087746 hasRelatedWork W2134546617 @default.
- W3149087746 hasRelatedWork W2148201750 @default.
- W3149087746 hasRelatedWork W2944042845 @default.
- W3149087746 hasRelatedWork W2969581410 @default.
- W3149087746 hasRelatedWork W2976642235 @default.
- W3149087746 hasRelatedWork W3023162102 @default.
- W3149087746 hasRelatedWork W1653780940 @default.
- W3149087746 hasRelatedWork W2107950834 @default.
- W3149087746 hasRelatedWork W2253766815 @default.
- W3149087746 hasRelatedWork W2873077872 @default.
- W3149087746 isParatext "false" @default.
- W3149087746 isRetracted "false" @default.
- W3149087746 magId "3149087746" @default.
- W3149087746 workType "article" @default.