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- W31492021 abstract "The growth and production of ©?-amylase and amyloglucosidaseby Aspergillus awamori NRRL 3112 and A. niger CBS 26265 wereinvestigated, with a view to obtaining optimal levels ofboth these enzymes in shake flask culture, and subsequently on the large scale.The effect of various parameters, such as carbon source,nitrogen source, presence/absence of buffering agents,carbon:nitrogen ratio , pH values, and time were examined.Following the screening of ten strains of Aspergilli, A.awamori NRRL 3112 was found to be the superioramyloglucosidase producing strain , while A. niger CBS 26265, produced higher levels of o i-amylase. The favoured nitrogen source for amylolytic enzyme production was corn steep powder, which had a strong buffering effecton the medium. Starch or its breakdown products stimulated ©?»amylase and amylolucosidase expression'as well as secretion. oC-Amylase production was more sensitive to pH than amyloglucosidase. The former showed peak production after 24-48 hours while the rate of amyloglucosidase synthesis was linear for 6-7 days.The fermentation was scaled up to 5 and 10 1 laboratoryscale fermenters. The effects of various agitation and aeration rates were examined. Oxygen transferrate (OTR) or oxygen transfercoefficient (K. a) was found to have a large effect on biomass and enzyme yield s , with the conditions for maximal amylase production corresponding to suboptimal conditions for Aspergillus growth.Controlling pH a t 4.5 or 5-5 increased oo-amylase production, but had little effect on amyloglucosidase yield s . The average doubling time of A. awamori NRRL 3112 varied with the medium pH. Minimum values were recorded a t pHi 4.1.The specific growth rate of this filamentous organismreached a maximum at 12 - 16 hours, a t 0.085-0.377 h1.This corresponded with the period of greatest change in pH.It is concluded that A. awamori NRRL 3112 is a superiorproducer of amyloglucosidase, and is most suitab le for large scale production of amylolytic enzymes." @default.
- W31492021 created "2016-06-24" @default.
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- W31492021 date "1987-01-01" @default.
- W31492021 modified "2023-09-24" @default.
- W31492021 title "Growth and production of amylotic enzymes by aspergillus spp" @default.
- W31492021 hasPublicationYear "1987" @default.
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