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- W3149206175 abstract "目的 探讨人参皂苷Rg1在造血干/祖细胞（HSC/HPC）连续移植中对抗细胞衰老的作用与去乙酰化酶6/核因子-κB（SIRT6/NF-κB）信号轴的关系。方法 免疫磁性分选法分离纯化雄性供体小鼠干细胞抗原阳性（Sca-1＋）HSC/HPC,连续移植3代构建HSC/HPC衰老体内模型。60Coγ射线致死剂量辐射雌性受体鼠后分4组,照射对照组;衰老模型组;Rg1治疗衰老组;Rg1预防衰老组。造血祖细胞混合集落（CFU-Mix）培养,细胞周期分析和衰老相关β-半乳糖苷酶（SA-β-Gal）染色分析Rg1体内调控Sca-1＋HSC/HPC衰老的作用。实时定量PCR及Western blotting检测衰老调控分子SIRT6、NF-κB mRNA及蛋白的表达。结果 连续移植后受体鼠Sca-1＋HSC/HPC出现细胞衰老特征,随移植代数的增加,Sca-1＋HSC/HPC G0/G1期细胞比例及SA-β-Gal染色阳性率增高,CFU-Mix数量下降。与同代衰老模型组相比,Rg1治疗衰老组及Rg1预防衰老组受体鼠Sca-1＋HSC/HPC G0/G1期细胞比例、SA-β-Gal染色阳性率下降,CFU-Mix数量升高;SIRT6 mRNA及蛋白表达上调,NF-κB mRNA及蛋白表达下调;Rg1预防衰老组各指标变化均较Rg1治疗衰老组明显。结论 Rg1可能通过调控SIRT6/NF-κB信号轴发挥其对抗连续移植过程中Sca-1＋HSC/HPC衰老的作用。" @default.
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- W3149206175 date "2015-01-01" @default.
- W3149206175 modified "2023-10-17" @default.
- W3149206175 title "人参皂苷Rg1在造血干/祖细胞连续移植中对延缓细胞衰老的作用与去乙酰化酶6/核因子-κB信号轴的关系" @default.
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