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- W3149223501 abstract "To establish Hp/DNA multi-parameter flow cytometry in order to analyze the relationship between cell cycel, apoptosis and the expression of Hp(haptoglobin) n bone marrow cells of radiation-injured mice. Mouse bone mar row cels were fixed with 70% ethanol and permeated by Triton_X100. Hp in the cells was indirectly labeled by immun of luroes cence while DNA in the cells was labeled by PI. Then them mous ebone marrown cells were valuated and analyzed by flow cytometry. Cell cycle and apoptosi of Hp~(+) or Hp~(-) cells were analyzed by using ModFit software with two gate circled. The ratio of Hp~(+) cells was determined by CELLQuest software. The proportion of Hp~(+) cells in nucleated cells deried from moues bone marrow was obviously increased at 6h after whle-boy #gamma#-irradiaton compared with that of the unirradiated control. Apoptosis happened in Hp~(+) cells of mouse bone marrow cells, but there was no apoptosi in Hp~(-) cells. G_(2)/M arrest was more obvious in Hp~(-) cells sthan in Hp~(+) cells. This informaiton can note inferred by merely using Wstern blotting or DNA analysis. Hp/DNA multi-parameter flow cytomertry can be applied to analysis of the relationship between cells cycle, apoptosis and the expression of Hp, and it is a quick and kojective analysis method." @default.
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- W3149223501 date "2004-01-01" @default.
- W3149223501 modified "2023-09-23" @default.
- W3149223501 title "Analysis of cell cycle, apoptosis and expression of Hp in bone marrow cells of acute radiaton-injured mice by Hp/DNA multi-parameter flow cytometry" @default.
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