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- W3149254876 abstract "有害 cyanobacterial 花蕾是一变得环境在自然的水里世界范围的问题， biodegradation 被发现是为移开有害 cyanobacteria 生产的 microcystins (MC ) 的最有效的方法。基于 Sphingopyxis sp 的有希望的细菌的紧张的隔离。为 biodegrading MC 的 USTB-05，我们第一次克隆并且表示在 E 为在 microcystin LR (MC-LR ) 的 biodegradation 的第一步负责的基因 USTB-05-A (HM245411 ) 。coli DH5 与容易的 pGEM-T 的克隆的向量和 pGEX-4T-1 的表示向量，分别地。recombinant E 的没有房间的摘录(CE ) 。包含 USTB-05-A 的 coli DH5 为 biodegrading MC-LR 举办了高活动。40 mg/L 的起始的 MC-LR 集中是完全在 1 hr 以内的 biodegraded 面对有 0.35 mg/mL 的蛋白质集中的 CE。基于液体色层谱团光谱(最小公倍数)的分析，基因 USTB-05-A 编码的酶被发现在劈开在 3-amino-9-methoxy-2,6 ， 8-trimethyl-10-phenyl-deca-4,6-dienoic 酸( Adda )和 MC-LR 的精氨酸之间的目标肽契约活跃，并且作为比更不有毒的第一个产品把周期的 MC-LR 变换成线性 MC-LR 父母 MC-LR ，它由 Sphingopyxis sp 在 MC-LR biodegradation 的小径上为第一步提供了直接证据。USTB-05。" @default.
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- W3149254876 date "2012-01-01" @default.
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- W3149254876 title "Cloning and expression of the first gene for biodegrading microcystin LR by Sphingopyxis sp. USTB-05" @default.
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