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• W3149258112 abstract "Abstract Because either trough or peak concentration at 2 h after administration is measured in routine therapeutic drug monitoring for cyclosporine A (CyA), a quantification method with a wide‐range calibration curve capable of simultaneously measuring both concentrations is required. We developed a sensitive, wide‐range and high‐throughput quantification method for CyA in whole blood using ultra‐performance liquid chromatography–tandem mass spectrometry (UPLC–MS/MS), and compared patients’ blood CyA levels measured by UPLC–MS/MS and antibody‐conjugated magnetic immunoassay (ACMIA). Whole blood samples were prepared by solid‐phase extraction using Oasis HLB μElution plate. The UPLC–MS/MS assay showed excellent linearity over a wide calibration range of 5–2500 ng/mL. Within‐batch accuracy and precision as well as batch‐to‐batch accuracy and precision fulfilled the criteria of US Food and Drug Administration guidelines. The blood CyA concentrations measured by the UPLC–MS/MS assay correlated strongly with those measured by ACMIA. A Bland–Altman plot showed a fixed error between CyA concentrations measured by the two methods, and the concentrations measured by the UPLC–MS/MS method were consistently lower than those measured by ACMIA. We have succeeded to develop a sensitive, wide‐range and high‐throughput quantification method for CyA in whole blood using UPLC–MS/MS." @default.
• W3149258112 created "2021-04-13" @default.
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• W3149258112 date "2021-04-19" @default.
• W3149258112 modified "2023-10-18" @default.
• W3149258112 title "Sensitive, wide‐range and high‐throughput quantification of cyclosporine in whole blood using ultra‐performance liquid chromatography coupled to tandem mass spectrometry and comparison with an antibody‐conjugated magnetic immunoassay" @default.
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• W3149258112 doi "https://doi.org/10.1002/bmc.5128" @default.
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• W3149258112 hasPublicationYear "2021" @default.
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