FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W3149259670> ?p ?o ?g. }

• W3149259670 endingPage "111446" @default.
• W3149259670 startingPage "111446" @default.
• W3149259670 abstract "Artificial metalloenzymes result from the insertion of a catalytically active metal complex into a biological scaffold, generally a protein devoid of other catalytic functionalities. As such, their design requires efforts to engineer substrate binding, in addition to accommodating the artificial catalyst. Here we constructed and characterised artificial metalloenzymes using alcohol dehydrogenase as starting point, an enzyme which has both a cofactor and a substrate binding pocket. A docking approach was used to determine suitable positions for catalyst anchoring to single cysteine mutants, leading to an artificial metalloenzyme capable to reduce both natural cofactors and the hydrophobic 1-benzylnicotinamide mimic. Kinetic studies revealed that the new construct displayed a Michaelis-Menten behaviour with the native nicotinamide cofactors, which were suggested by docking to bind at a surface exposed site, different compared to their native binding position. On the other hand, the kinetic and docking data suggested that a typical enzyme behaviour was not observed with the hydrophobic 1-benzylnicotinamide mimic, with which binding events were plausible both inside and outside the protein. This work demonstrates an extended substrate scope of the artificial metalloenzymes and provides information about the binding sites of the nicotinamide substrates, which can be exploited to further engineer artificial metalloenzymes for cofactor regeneration. SYNOPSIS ABOUT GRAPHICAL ABSTRACT: The manuscript provides information on the design of artificial metalloenzymes based on the bioconjugation of rhodium complexes to alcohol dehydrogenase, to improve their ability to reduce hydrophobic substrates. The graphical abstract presents different binding modes and results observed with native cofactors as substrates, compared to the hydrophobic benzylnicotinamide." @default.
• W3149259670 created "2021-04-13" @default.
• W3149259670 creator A5011866867 @default.
• W3149259670 creator A5018897067 @default.
• W3149259670 creator A5053983913 @default.
• W3149259670 creator A5057504207 @default.
• W3149259670 creator A5061431167 @default.
• W3149259670 creator A5082869226 @default.
• W3149259670 date "2021-07-01" @default.
• W3149259670 modified "2023-10-18" @default.
• W3149259670 title "Design of artificial metalloenzymes for the reduction of nicotinamide cofactors" @default.
• W3149259670 cites W1757079284 @default.
• W3149259670 cites W1956612320 @default.
• W3149259670 cites W1966758619 @default.
• W3149259670 cites W1974542238 @default.
• W3149259670 cites W1985526606 @default.
• W3149259670 cites W1990195346 @default.
• W3149259670 cites W1990916863 @default.
• W3149259670 cites W2005871028 @default.
• W3149259670 cites W2019849550 @default.
• W3149259670 cites W2025459439 @default.
• W3149259670 cites W2088393328 @default.
• W3149259670 cites W2141556222 @default.
• W3149259670 cites W2151693483 @default.
• W3149259670 cites W2222589778 @default.
• W3149259670 cites W2229505963 @default.
• W3149259670 cites W2237408143 @default.
• W3149259670 cites W2330799739 @default.
• W3149259670 cites W2340795529 @default.
• W3149259670 cites W2342711514 @default.
• W3149259670 cites W2560551130 @default.
• W3149259670 cites W2588361971 @default.
• W3149259670 cites W2599859268 @default.
• W3149259670 cites W2618860683 @default.
• W3149259670 cites W2735839143 @default.
• W3149259670 cites W2779551360 @default.
• W3149259670 cites W2885747247 @default.
• W3149259670 cites W2896514107 @default.
• W3149259670 cites W2911989070 @default.
• W3149259670 cites W2912459103 @default.
• W3149259670 cites W2915890876 @default.
• W3149259670 cites W2919396331 @default.
• W3149259670 cites W2930575615 @default.
• W3149259670 doi "https://doi.org/10.1016/j.jinorgbio.2021.111446" @default.
• W3149259670 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/33865209" @default.
• W3149259670 hasPublicationYear "2021" @default.
• W3149259670 type Work @default.
• W3149259670 sameAs 3149259670 @default.
• W3149259670 citedByCount "9" @default.
• W3149259670 countsByYear W31492596702021 @default.
• W3149259670 countsByYear W31492596702022 @default.
• W3149259670 countsByYear W31492596702023 @default.
• W3149259670 crossrefType "journal-article" @default.
• W3149259670 hasAuthorship W3149259670A5011866867 @default.
• W3149259670 hasAuthorship W3149259670A5018897067 @default.
• W3149259670 hasAuthorship W3149259670A5053983913 @default.
• W3149259670 hasAuthorship W3149259670A5057504207 @default.
• W3149259670 hasAuthorship W3149259670A5061431167 @default.
• W3149259670 hasAuthorship W3149259670A5082869226 @default.
• W3149259670 hasBestOaLocation W31492596702 @default.
• W3149259670 hasConcept C107824862 @default.
• W3149259670 hasConcept C111368507 @default.
• W3149259670 hasConcept C127313418 @default.
• W3149259670 hasConcept C147816474 @default.
• W3149259670 hasConcept C159110408 @default.
• W3149259670 hasConcept C171250308 @default.
• W3149259670 hasConcept C181199279 @default.
• W3149259670 hasConcept C185592680 @default.
• W3149259670 hasConcept C192562407 @default.
• W3149259670 hasConcept C197957613 @default.
• W3149259670 hasConcept C21951064 @default.
• W3149259670 hasConcept C2777289219 @default.
• W3149259670 hasConcept C41183919 @default.
• W3149259670 hasConcept C41685203 @default.
• W3149259670 hasConcept C53105671 @default.
• W3149259670 hasConcept C55493867 @default.
• W3149259670 hasConcept C71240020 @default.
• W3149259670 hasConcept C71924100 @default.
• W3149259670 hasConceptScore W3149259670C107824862 @default.
• W3149259670 hasConceptScore W3149259670C111368507 @default.
• W3149259670 hasConceptScore W3149259670C127313418 @default.
• W3149259670 hasConceptScore W3149259670C147816474 @default.
• W3149259670 hasConceptScore W3149259670C159110408 @default.
• W3149259670 hasConceptScore W3149259670C171250308 @default.
• W3149259670 hasConceptScore W3149259670C181199279 @default.
• W3149259670 hasConceptScore W3149259670C185592680 @default.
• W3149259670 hasConceptScore W3149259670C192562407 @default.
• W3149259670 hasConceptScore W3149259670C197957613 @default.
• W3149259670 hasConceptScore W3149259670C21951064 @default.
• W3149259670 hasConceptScore W3149259670C2777289219 @default.
• W3149259670 hasConceptScore W3149259670C41183919 @default.
• W3149259670 hasConceptScore W3149259670C41685203 @default.
• W3149259670 hasConceptScore W3149259670C53105671 @default.
• W3149259670 hasConceptScore W3149259670C55493867 @default.
• W3149259670 hasConceptScore W3149259670C71240020 @default.
• W3149259670 hasConceptScore W3149259670C71924100 @default.
• W3149259670 hasFunder F4320334629 @default.
• W3149259670 hasLocation W31492596701 @default.

• next