FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W3149389613> ?p ?o ?g. }

• W3149389613 abstract "One of the main obstacles for systematic evolution of ligands by exponential enrichment (SELEX) failure is the generation of a non-specific product, as selection-inherent amplification procedures tend to form by-products, which prevents the enrichment of target-binding aptamers. Herein, we reported a dual-microfluidic amplified system (dual-MAS) based on the real-time polymerase chain reaction (PCR) detection chip and the large volume PCR chip for one-step specific PCR and for evaluating the SELEX process. First, it is a simple method to accomplish analytical PCR and amplification PCR in one step, and the optimal number of cycles for generating the specific PCR product is the cycles when the slope of the linear amplification period of the real-time PCR curve begins to decrease. Second, the time used by the dual-MAS for generating a specific PCR product is reduced to 30 min, and the multi-functional dual-MAS can simultaneously evaluate the SELEX process by providing important information on the amounts of enriched sequences and the library diversity in every round of SELEX. In addition, pollution contamination and fragment loss can be significantly avoided in the closed chip. Last, the specific PCR product, the amounts of enriched sequences, and the library diversity can be obtained for every single SELEX in just 30 min. Compared with current methods, this system can reduce the time for generating a specific PCR product and SELEX, and it is easier to choose the optimal number of cycles for a specific PCR product. In a word, it is a sensitive, simple, and rapid strategy to improve the specificity of the PCR product and make the process of SELEX in a controlled way." @default.
• W3149389613 created "2021-04-13" @default.
• W3149389613 creator A5007292122 @default.
• W3149389613 creator A5048379858 @default.
• W3149389613 creator A5054390905 @default.
• W3149389613 creator A5055187624 @default.
• W3149389613 creator A5064432785 @default.
• W3149389613 date "2021-03-01" @default.
• W3149389613 modified "2023-10-12" @default.
• W3149389613 title "Accomplishment of one-step specific PCR and evaluated SELEX process by a dual-microfluidic amplified system" @default.
• W3149389613 cites W1983955838 @default.
• W3149389613 cites W1999599455 @default.
• W3149389613 cites W2021866867 @default.
• W3149389613 cites W2035285327 @default.
• W3149389613 cites W2043137047 @default.
• W3149389613 cites W2044601776 @default.
• W3149389613 cites W2046309447 @default.
• W3149389613 cites W2059281433 @default.
• W3149389613 cites W2072461007 @default.
• W3149389613 cites W2074159527 @default.
• W3149389613 cites W2079321730 @default.
• W3149389613 cites W2101326349 @default.
• W3149389613 cites W2105398927 @default.
• W3149389613 cites W2117904651 @default.
• W3149389613 cites W2136004642 @default.
• W3149389613 cites W2138141188 @default.
• W3149389613 cites W2330503778 @default.
• W3149389613 cites W2513851882 @default.
• W3149389613 cites W2547782348 @default.
• W3149389613 cites W2552828794 @default.
• W3149389613 cites W2736861854 @default.
• W3149389613 cites W2792169093 @default.
• W3149389613 cites W2906364404 @default.
• W3149389613 cites W2964120378 @default.
• W3149389613 cites W2970007078 @default.
• W3149389613 cites W2982242066 @default.
• W3149389613 cites W3017193580 @default.
• W3149389613 doi "https://doi.org/10.1063/5.0045965" @default.
• W3149389613 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/8024032" @default.
• W3149389613 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/33841601" @default.
• W3149389613 hasPublicationYear "2021" @default.
• W3149389613 type Work @default.
• W3149389613 sameAs 3149389613 @default.
• W3149389613 citedByCount "0" @default.
• W3149389613 crossrefType "journal-article" @default.
• W3149389613 hasAuthorship W3149389613A5007292122 @default.
• W3149389613 hasAuthorship W3149389613A5048379858 @default.
• W3149389613 hasAuthorship W3149389613A5054390905 @default.
• W3149389613 hasAuthorship W3149389613A5055187624 @default.
• W3149389613 hasAuthorship W3149389613A5064432785 @default.
• W3149389613 hasBestOaLocation W31493896132 @default.
• W3149389613 hasConcept C104317684 @default.
• W3149389613 hasConcept C122682173 @default.
• W3149389613 hasConcept C128972844 @default.
• W3149389613 hasConcept C153911025 @default.
• W3149389613 hasConcept C171250308 @default.
• W3149389613 hasConcept C192562407 @default.
• W3149389613 hasConcept C41008148 @default.
• W3149389613 hasConcept C49105822 @default.
• W3149389613 hasConcept C54355233 @default.
• W3149389613 hasConcept C67705224 @default.
• W3149389613 hasConcept C70721500 @default.
• W3149389613 hasConcept C8673954 @default.
• W3149389613 hasConcept C86803240 @default.
• W3149389613 hasConceptScore W3149389613C104317684 @default.
• W3149389613 hasConceptScore W3149389613C122682173 @default.
• W3149389613 hasConceptScore W3149389613C128972844 @default.
• W3149389613 hasConceptScore W3149389613C153911025 @default.
• W3149389613 hasConceptScore W3149389613C171250308 @default.
• W3149389613 hasConceptScore W3149389613C192562407 @default.
• W3149389613 hasConceptScore W3149389613C41008148 @default.
• W3149389613 hasConceptScore W3149389613C49105822 @default.
• W3149389613 hasConceptScore W3149389613C54355233 @default.
• W3149389613 hasConceptScore W3149389613C67705224 @default.
• W3149389613 hasConceptScore W3149389613C70721500 @default.
• W3149389613 hasConceptScore W3149389613C8673954 @default.
• W3149389613 hasConceptScore W3149389613C86803240 @default.
• W3149389613 hasFunder F4320321001 @default.
• W3149389613 hasIssue "2" @default.
• W3149389613 hasLocation W31493896131 @default.
• W3149389613 hasLocation W31493896132 @default.
• W3149389613 hasLocation W31493896133 @default.
• W3149389613 hasOpenAccess W3149389613 @default.
• W3149389613 hasPrimaryLocation W31493896131 @default.
• W3149389613 hasRelatedWork W1487881598 @default.
• W3149389613 hasRelatedWork W2011367153 @default.
• W3149389613 hasRelatedWork W2099207551 @default.
• W3149389613 hasRelatedWork W2151839185 @default.
• W3149389613 hasRelatedWork W2162977224 @default.
• W3149389613 hasRelatedWork W2287289200 @default.
• W3149389613 hasRelatedWork W2294681031 @default.
• W3149389613 hasRelatedWork W2934274357 @default.
• W3149389613 hasRelatedWork W2944604860 @default.
• W3149389613 hasRelatedWork W2952517342 @default.
• W3149389613 hasVolume "15" @default.
• W3149389613 isParatext "false" @default.
• W3149389613 isRetracted "false" @default.
• W3149389613 magId "3149389613" @default.
• W3149389613 workType "article" @default.