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- W3149397412 abstract "An efficient protocol for plant regeneration was developed from protoplasts of Gentiana macrophylla Pall. through somatic embryogenesis. Viable protoplasts were isolated from cell suspensions derived from young seedling leaves in an enzyme solution containing 2 % Cellulase Ono- zuka R-10, 0.5 % Macerozyme R-10, 0.5 % Hemicellulase, and 0.4 M sorbitol with a yield of 6.2 9 10 6 protoplasts g -1 fresh weight. Liquid, solid-liquid double layer (sLD) and agar-pool (aPL) culture systems were used for protoplast culture. The frequency of protoplast cell divisions and colony formations in aPL culture were significant (p 0.05) higher than those in liquid and sLD cultures. The highest division frequency and plating efficiency were 37.7 % and 16.5 %, respectively, in aPL culture supplemented with 2 mg l -1 2,4- dichlorophenoxyacetic acid (2,4-D )a nd 0.5 mg l -1 6-ben- zylaminopurine (BA). Protoplast-derived microcalli obtained from aPL culture system were transferred to solid MS medium with a reduced concentration of 2,4-D (0.5 mg l -1 )t o promote the formation of embryogenic calli. Somatic embryos devel- oped into plantlets on MS medium supplemented with 2m g l -1 BA at a rate of 51.3 %. RAPD analysis of G. mac-" @default.
- W3149397412 created "2021-04-13" @default.
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- W3149397412 date "2015-01-01" @default.
- W3149397412 modified "2023-09-27" @default.
- W3149397412 title "Plant regeneration from protoplasts of Gentiana macrophylla Pall. using agar-pool culture" @default.
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